AB250764

Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SNAP-beta antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SNAPB, NAPB, Beta-soluble NSF attachment protein, SNAP-beta, N-ethylmaleimide-sensitive factor attachment protein beta

3 Images

Supplier Data

Immunoprecipitation - Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (AB250764)

This data was developed using ab184563, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab184563 at 1/50 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/snap-beta-antibody-epr15046b-n-terminal-ab184563'>ab184563</a>)

Predicted band size: 34 kDa

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Supplier Data

Western blot - Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (AB250764)

This data was developed using ab184563, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/snap-beta-antibody-epr15046b-n-terminal-ab184563'>ab184563</a>) at 1/5000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

true

Supplier Data

Western blot - Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (AB250764)

This data was developed using ab184563, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/snap-beta-antibody-epr15046b-n-terminal-ab184563'>ab184563</a>) at 1/5000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 34 kDa

true

Unconjugated

Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15046(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250764 is the carrier-free version of ab184563.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP-beta also known as SNAP-23 is part of the SNARE family consisting of about 23 kDa proteins. This protein plays a role in vesicular transport processes working in the docking and fusion of vesicles with membranes. Expressed broadly in mammalian tissues SNAP-beta shows higher levels in brain and adipose tissue indicating its involvement in vital cellular functions across various organs.

Biological function summary

SNAP-beta contributes to the regulation of membrane trafficking essential for processes such as neurotransmitter release and insulin-mediated glucose uptake. It acts as a member of the SNARE complex alongside syntaxin and VAMP proteins facilitating membrane fusion within cells. This functional integration within a complex highlights its significance in efficient intracellular communication.

Pathways

Vesicular transport and exocytosis highlight the overlapping roles of SNAP-beta within the cell. SNAP-beta engages in these pathways by interacting with proteins like syntaxin 4 and synaptobrevin. This linkage ensures precise membrane fusion events necessary for effective cellular responses and functions.

SNAP-beta has associations with type 2 diabetes and neurological disorders. Altered SNAP-beta function can cause disruptions in insulin signaling and neurotransmitter release contributing to these conditions. It often interacts with proteins like GLUT4 affecting glucose metabolism and synaptic transmission underlining its involvement in related pathophysiological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.

See full target information NAPB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free

Immunoprecipitation - Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (AB250764)

Western blot - Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (AB250764)

Western blot - Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (AB250764)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise