JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131242

Anti-SNAP23 antibody [EPR8538]

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal SNAP23 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Synaptosomal-associated protein 23, SNAP-23, Vesicle-membrane fusion protein SNAP-23, SNAP23

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] (AB131242)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] (AB131242)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SNAP23 with ab131242 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SNAP23 antibody [EPR8538] (AB131242)
  • WB

Lab

Western blot - Anti-SNAP23 antibody [EPR8538] (AB131242)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : SNAP23 knockout HAP1 cell lysate (20 μg)
Lane 3 : HEK293 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab131242 observed at 25 kDa. Red - loading control, ab8245, observed at 37kDa.
ab131242 was shown to recognize SNAP23 when SNAP23 knockout samples were used, along with additional cross-reactive bands. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab131242 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SNAP23 antibody [EPR8538] (ab131242)

Predicted band size: 23 kDa

false

Western blot - Anti-SNAP23 antibody [EPR8538] (AB131242)
  • WB

Unknown

Western blot - Anti-SNAP23 antibody [EPR8538] (AB131242)

All lanes:

Western blot - Anti-SNAP23 antibody [EPR8538] (ab131242) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human placenta cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

HepG2 cell lysat at 10 µg

Lane 5:

JAR cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

false

OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] (AB131242)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] (AB131242)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free

  • Carrier free

    Anti-SNAP23 antibody [EPR8538] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8538

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB229085

Anti-SNAP23 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (AB229085)

  • 0
  • 0
Primary Antibodies

AB3340

Anti-SNAP23 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (AB3340)

  • 4
  • 7
Primary Antibodies

AB4114

Anti-SNAP23 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (AB4114)

  • 4
  • 2
Primary Antibodies

AB133703

Anti-SNAP23 antibody [EPR8539]

RabMAb|Recombinant|KO Validated

Western blot - Anti-SNAP23 antibody [EPR8539] (AB133703)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB133750

Anti-Syntaxin 3 antibody [EPR8543]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 3 antibody [EPR8543] (AB133750)

  • 5
  • 2
Primary Antibodies

AB184545

Anti-Syntaxin 4 antibody [EPR15473]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Syntaxin 4 antibody [EPR15473] (AB184545)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109009

Anti-Rab4 antibody [EPR3043] - Early Endosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (AB109009)

  • 1
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP23 also known as Synaptosomal-Associated Protein 23 is an important component in the mechanism of vesicular trafficking. It has a molecular mass of approximately 23 kDa and functions as a t-SNARE (target membrane SNAP receptor) protein. SNAP23 is expressed in various tissues with high expression levels in the brain pancreas liver and adipose tissue. This protein facilitates the fusion of vesicles with target membranes by forming SNARE complexes which are essential for the exocytosis and endocytosis processes.
Biological function summary

SNAP23 plays a significant role in the regulation of membrane fusion events and intracellular transport. It forms part of the SNARE complex which includes v-SNAREs on vesicle membranes and other t-SNAREs on target membranes. This complex orchestrates the docking and fusion of vesicles enabling the release of neurotransmitters and other cargoes in neuronal and non-neuronal cells. By mediating these events SNAP23 is important for neurotransmission insulin-regulated glucose uptake and other cellular processes.

Pathways

SNAP23 functions in the exocytotic and endocytic pathways where it aids in the accurate secretion and recycling of cellular components. It interacts with proteins like Syntaxin and VAMP (vesicle-associated membrane protein) to achieve precise vesicle targeting and fusion. SNAP23 is also implicated in insulin-signaling pathways where it modulates GLUT4 translocation to the cell surface a process vital for maintaining glucose homeostasis. These interactions underline its involvement in both neural communication and metabolic regulation.

SNAP23 has been associated with metabolic conditions such as type 2 diabetes and neurological disorders. The protein's function in insulin-regulated glucose transport suggests its relevance in diabetes where impaired glucose uptake occurs. In neurological contexts alterations in SNAP23 expression or function might contribute to disorders affecting synaptic transmission. Additionally its connection with proteins like GLUT4 and VAMP highlights SNAP23's role in disease contexts providing a linkage between vesicular transport and disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
See full target information Synaptosomal-associated protein 23
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Virology 578:117-127 PubMed36527930

2022

SNAP23 is essential for germination of EV-D68 replication organelles.

Applications

Unspecified application

Species

Unspecified reactive species

Katelyn Miller,Michael A Wagner,Alagie Jassey,William T Jackson

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen

Gene 673:217-224 PubMed29908998

2018

SNAP23 suppresses cervical cancer progression via modulating the cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Biqing Zhu,Quanli Zhang,Yaqin Wu,Jing Luo,Xiufen Zheng,Lin Xu,Emei Lu,Junwei Qu,Binhui Ren

Journal of virology 91: PubMed28356525

2017

MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2.

Applications

WB

Species

Human

Mitsuhiro Machitani,Fuminori Sakurai,Keisaku Wakabayashi,Kosuke Nakatani,Masashi Tachibana,Hiroyuki Mizuguchi

The EMBO journal 36:42-60 PubMed27932448

2016

Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Kimura,Jingyue Jia,Suresh Kumar,Seong Won Choi,Yuexi Gu,Michal Mudd,Nicolas Dupont,Shanya Jiang,Ryan Peters,Farzin Farzam,Ashish Jain,Keith A Lidke,Christopher M Adams,Terje Johansen,Vojo Deretic
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com