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AB248624

Anti-SNAP23 antibody [EPR8539] - BSA and Azide free

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Rabbit Recombinant Monoclonal SNAP23 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

Synaptosomal-associated protein 23, SNAP-23, Vesicle-membrane fusion protein SNAP-23, SNAP23

3 Images
Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (AB248624)
  • WB

Lab

Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (AB248624)

This data was developed using ab133703, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : SNAP23 knockout HAP1 cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab133703 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133703 was shown to specifically react with SNAP23 when SNAP23 knockout samples were used. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab133703 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SNAP23 antibody [EPR8539] (<a href='/en-us/products/primary-antibodies/snap23-antibody-epr8539-ab133703'>ab133703</a>)

Predicted band size: 23 kDa

false

Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (AB248624)
  • WB

Unknown

Western blot - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (AB248624)

This data was developed using ab133703, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNAP23 antibody [EPR8539] (<a href='/en-us/products/primary-antibodies/snap23-antibody-epr8539-ab133703'>ab133703</a>) at 1/1000 dilution

Lane 1:

Fetal liver cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

BxPC-3 cell lysate

Predicted band size: 23 kDa

false

OI-RD Scanning - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (AB248624)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SNAP23 antibody [EPR8539] - BSA and Azide free (AB248624)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8539

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248624 is the carrier-free version of ab133703.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP23 also known as Synaptosomal-Associated Protein 23 is an important component in the mechanism of vesicular trafficking. It has a molecular mass of approximately 23 kDa and functions as a t-SNARE (target membrane SNAP receptor) protein. SNAP23 is expressed in various tissues with high expression levels in the brain pancreas liver and adipose tissue. This protein facilitates the fusion of vesicles with target membranes by forming SNARE complexes which are essential for the exocytosis and endocytosis processes.
Biological function summary

SNAP23 plays a significant role in the regulation of membrane fusion events and intracellular transport. It forms part of the SNARE complex which includes v-SNAREs on vesicle membranes and other t-SNAREs on target membranes. This complex orchestrates the docking and fusion of vesicles enabling the release of neurotransmitters and other cargoes in neuronal and non-neuronal cells. By mediating these events SNAP23 is important for neurotransmission insulin-regulated glucose uptake and other cellular processes.

Pathways

SNAP23 functions in the exocytotic and endocytic pathways where it aids in the accurate secretion and recycling of cellular components. It interacts with proteins like Syntaxin and VAMP (vesicle-associated membrane protein) to achieve precise vesicle targeting and fusion. SNAP23 is also implicated in insulin-signaling pathways where it modulates GLUT4 translocation to the cell surface a process vital for maintaining glucose homeostasis. These interactions underline its involvement in both neural communication and metabolic regulation.

SNAP23 has been associated with metabolic conditions such as type 2 diabetes and neurological disorders. The protein's function in insulin-regulated glucose transport suggests its relevance in diabetes where impaired glucose uptake occurs. In neurological contexts alterations in SNAP23 expression or function might contribute to disorders affecting synaptic transmission. Additionally its connection with proteins like GLUT4 and VAMP highlights SNAP23's role in disease contexts providing a linkage between vesicular transport and disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
See full target information Synaptosomal-associated protein 23
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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