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AB66066

Anti-SNAP25 antibody [4A3]

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(10 Publications)

Mouse Monoclonal SNAP25 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Zebrafish samples. Cited in 10 publications.

View Alternative Names

SNAP, SNAP25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [4A3] (AB66066)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [4A3] (AB66066)

ab66066 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66066 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-SNAP25 antibody [4A3] (AB66066)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-SNAP25 antibody [4A3] (AB66066)

Overlay histogram showing SH-SY5Y cells stained with ab66066 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66066, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

Western blot - Anti-SNAP25 antibody [4A3] (AB66066)
  • WB

Unknown

Western blot - Anti-SNAP25 antibody [4A3] (AB66066)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-SNAP25 antibody [4A3] (ab66066) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Zebrafish brain homogenate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µg

Lane 4:

Mouse brain homogenate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

true

Exposure time: 2min

Western blot - Anti-SNAP25 antibody [4A3] (AB66066)
  • WB

Unknown

Western blot - Anti-SNAP25 antibody [4A3] (AB66066)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-SNAP25 antibody [4A3] (ab66066) at 1 µg/mL

Lane 1:

SNAP25 transfected 293T cell lysate at 50 µg

Lane 2:

Non-transfected 293T cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4A3

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Human, Zebrafish

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Opossum": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
See full target information SNAP25
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:3368-3385 PubMed40093888

2025

Sex- and age-specific sensitivities of the endocannabinoid system in Alzheimer's disease revealed by PET imaging with [F]FMPEP- and [F]MAGL-2102.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Pees,Christopher Daniel Morrone,Junchao Tong,Jian Rong,Tuo Shao,Darcy Wear,Steven H Liang,Wai Haung Yu,Neil Vasdev

Frontiers in molecular neuroscience 16:1123343 PubMed36873105

2023

Alleviation of cognitive deficits in a rat model of glutamate-induced excitotoxicity, using an N-type voltage-gated calcium channel ligand, extracted from crude venom.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Keimasi,Kowsar Salehifard,Mohammadjavad Keimasi,Mohammadreza Amirsadri,Noushin Mirshah Jafar Esfahani,Majid Moradmand,Fariba Esmaeili,Mohammad Reza Mofid

The EMBO journal 41:e10242 PubMed35993331

2022

Tau target identification reveals NSF-dependent effects on AMPA receptor trafficking and memory formation.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel Prikas,Esmeralda Paric,Prita R Asih,Kristie Stefanoska,Holly Stefen,Thomas Fath,Anne Poljak,Arne Ittner

Movement disorders : official journal of the Movement Disorder Society 37:949-961 PubMed35420219

2022

Alpha-Synuclein is Involved in DYT1 Dystonia Striatal Synaptic Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Ponterio,Gaia Faustini,Ilham El Atiallah,Giuseppe Sciamanna,Maria Meringolo,Annalisa Tassone,Paola Imbriani,Silvia Cerri,Giuseppina Martella,Paola Bonsi,Arianna Bellucci,Antonio Pisani

Neuroscience 411:185-201 PubMed31158434

2019

Regulation of Synaptosomal GLT-1 and GLAST during Epileptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Allison R Peterson,Devin K Binder

Toxins 11: PubMed30791373

2019

Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Joussain,Olivier Le Coz,Andrey Pichugin,Peggy Marconi,Filip Lim,Mariaconcetta Sicurella,Andrea Salonia,Francesco Montorsi,Francisco Wandosell,Keith Foster,François Giuliano,Alberto L Epstein,Alejandro Aranda Muñoz

Neurobiology of disease 121:274-285 PubMed30347267

2018

PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by influencing synaptic function in the primary motor cortex of rats.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Mo,Bantong Wang,Xilin Zhu,Xiaopan Wu,Ying Liu

Neural plasticity 2017:4526417 PubMed29318050

2017

Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Mengjie Zhang,Jiahao Shi,Yunhe Zhou,Zhipeng Wan,Yicheng Wang,Yinghan Wan,Jun Li,Zhugang Wang,Jian Fei

Neuropharmacology 126:48-57 PubMed28844595

2017

Cognitive impairments associated with alterations in synaptic proteins induced by the genetic loss of adenosine A receptors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Moscoso-Castro,Marc López-Cano,Irene Gracia-Rubio,Francisco Ciruela,Olga Valverde

Scientific reports 7:9452 PubMed28842709

2017

The Parkinson's disease-associated GPR37 receptor interacts with striatal adenosine A receptor controlling its cell surface expression and function in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Morató,Rafael Luján,Marc López-Cano,Jorge Gandía,Igor Stagljar,Masahiko Watanabe,Rodrigo A Cunha,Víctor Fernández-Dueñas,Francisco Ciruela
View all publications
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