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AB31281

Anti-SNAP25 antibody

5

(1 Review)

|

(6 Publications)

Goat Polyclonal SNAP25 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SNAP25 aa 150-250.

View Alternative Names

SNAP, SNAP25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (AB31281)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (AB31281)

ab31281 negative control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (AB31281)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody (AB31281)

ab31281 (1μg/ml) staining of SNAP25 in paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SNAP25 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P60880

Specificity

This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.005-0.01 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.005-0.01 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.005-0.01 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
See full target information SNAP25
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40309958

2025

GPRC6A as a novel kokumi receptor responsible for enhanced taste preferences by ornithine.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yamamoto,Kayoko Ueji,Haruno Mizuta,Chizuko Inui-Yamamoto,Natsuko Kumamoto,Yasuhiro Shibata,Shinya Ugawa

International journal of molecular sciences 25: PubMed39201644

2024

Functional and Structural Changes in Diaphragm Neuromuscular Junctions in Early Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Andrei N Tsentsevitsky,Guzel V Sibgatullina,Yulia G Odoshivkina,Venera F Khuzakhmetova,Anna R Tokmakova,Anastasia A Ponomareva,Vadim V Salnikov,Guzalia F Zakirjanova,Alexey M Petrov,Ellya A Bukharaeva

International journal of molecular sciences 24: PubMed37240370

2023

Early Alterations in Structural and Functional Properties in the Neuromuscular Junctions of Mutant FUS Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marat A Mukhamedyarov,Aydar N Khabibrakhmanov,Venera F Khuzakhmetova,Arthur R Giniatullin,Guzalia F Zakirjanova,Nikita V Zhilyakov,Kamilla A Mukhutdinova,Dmitry V Samigullin,Pavel N Grigoryev,Andrey V Zakharov,Andrey L Zefirov,Alexey M Petrov

Nutrients 13: PubMed34836006

2021

Additive Effects of L-Ornithine on Preferences to Basic Taste Solutions in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haruno Mizuta,Natsuko Kumamoto,Shinya Ugawa,Takashi Yamamoto

Protein & cell 12:545-556 PubMed33548033

2021

Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Liang,Zhigang Xue,Jinfeng Xue,Duanyang Xie,Ke Xiong,Huixing Zhou,Fulei Zhang,Xuling Su,Guanghua Wang,Qicheng Zou,Yi Liu,Jian Yang,Honghui Ma,Luying Peng,Chunyu Zeng,Gang Li,Li Wang,Yi-Han Chen

The Journal of clinical investigation 123:646-56 PubMed23281397

2013

Deimination restores inner retinal visual function in murine demyelinating disease.

Applications

ELISA

Species

Rat

Mabel Enriquez-Algeciras,Di Ding,Fabrizio G Mastronardi,Robert E Marc,Vittorio Porciatti,Sanjoy K Bhattacharya
View all publications
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Product promise

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