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AB5666

Anti-SNAP25 antibody

5

(5 Reviews)

|

(62 Publications)

Rabbit Polyclonal SNAP25 antibody. Suitable for ICC, Flow Cyt, WB and reacts with Rat, Mouse samples. Cited in 62 publications. Immunogen corresponding to Synthetic Peptide within Mouse Snap25 aa 150-250.

View Alternative Names

Snap, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

4 Images
Immunocytochemistry - Anti-SNAP25 antibody (AB5666)
  • ICC

Unknown

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)

ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SNAP25 antibody (AB5666)

Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.

Western blot - Anti-SNAP25 antibody (AB5666)
  • WB

Supplier Data

Western blot - Anti-SNAP25 antibody (AB5666)

All lanes:

Western blot - Anti-SNAP25 antibody (ab5666)

Lane 1:

Mouse cerebellum at 30 µg

Lane 2:

Mouse brain at 30 µg

Lane 3:

Rat brain at 30 µg

Lane 4:

Membrane enriched extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate

Predicted band size: 23 kDa

false

Western blot - Anti-SNAP25 antibody (AB5666)
  • WB

AbReview11837****

Western blot - Anti-SNAP25 antibody (AB5666)

All lanes:

Western blot - Anti-SNAP25 antibody (ab5666) at 1/10000 dilution

All lanes:

Whole tissue lysate prepared from mouse hippocampus

Secondary

All lanes:

HRP conjugated goat anti-rabbit Ig at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

true

Exposure time: 20s

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, Flow Cyt, ICC

applications

Immunogen

Synthetic Peptide within Mouse Snap25 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P60879

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed : 8103915, PubMed : 8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed : 16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (By similarity).
See full target information Snap25
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Publications (62)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111156 PubMed39507243

2024

SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Vineeth Andisseryparambil Raveendran,Melissa Serranilla,Azam Asgarihafshejani,Miranda de Saint-Rome,Mariia Cherednychenko,Shanelle Mullany,Jennifer A Mitchell,Jessica C Pressey,Melanie A Woodin

EMBO molecular medicine 16:2795-2826 PubMed39402139

2024

Unraveling autophagic imbalances and therapeutic insights in Mecp2-deficient models.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Esposito,Tommaso Seri,Martina Breccia,Marzia Indrigo,Giuseppina De Rocco,Francesca Nuzzolillo,Vanna Denti,Francesca Pappacena,Gaia Tartaglione,Simone Serrao,Giuseppe Paglia,Luca Murru,Stefano de Pretis,Jean-Michel Cioni,Nicoletta Landsberger,Fabrizia Claudia Guarnieri,Michela Palmieri

NPJ Parkinson's disease 10:107 PubMed38773105

2024

A stem cell-based assay platform demonstrates alpha-synuclein dependent synaptic dysfunction in patient-derived cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J White,Karis A Clark,Kellianne D Alexander,Nagendran Ramalingam,Tracy L Young-Pearse,Ulf Dettmer,Dennis J Selkoe,Gary P H Ho

Biology 12: PubMed37372046

2023

Brain-Derived Estrogen Regulates Neurogenesis, Learning and Memory with Aging in Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Huang,Wuxiang Sun,Fujia Gao,Haoran Ma,Tao Yuan,Zixuan Liu,Huiyu Liu,Jiewei Hu,Jing Bai,Xin Zhang,Ruimin Wang

Science signaling 16:eadd7220 PubMed36787382

2023

Palmitoylation of the Parkinson's disease-associated protein synaptotagmin-11 links its turnover to α-synuclein homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gary P H Ho,Erin C Wilkie,Andrew J White,Dennis J Selkoe

The Journal of pharmacology and experimental therapeutics 383:117-128 PubMed36116796

2022

Metabotropic Glutamate Receptors Modulate Exocytotic Tau Release and Propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Mazzo,Ioana Butnaru,Olivera Grubisha,Elena Ficulle,Helen Sanger,Griffin Fitzgerald,Feng Pan,Francesca Pasqui,Tracey Murray,James Monn,Xia Li,Michael Hutton,Suchira Bose,Giampietro Schiavo,Emanuele Sher

ASN neuro 14:17590914221121257 PubMed36017573

2022

Exposure to Zika Virus Results in sex-Specific Memory Deficits and Neurological Alterations in Adult Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Thiago A Andrade,Julia S Fahel,Jessica M de Souza,Ana C Terra,Danielle G Souza,Vivian V Costa,Mauro M Teixeira,Enrrico Bloise,Fabiola M Ribeiro

Frontiers in neuroscience 16:905403 PubMed35860293

2022

Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

ShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin Li

Brain pathology (Zurich, Switzerland) 32:e13056 PubMed35178783

2022

Glial activation in prion diseases is selectively triggered by neuronal PrP.

Applications

Unspecified application

Species

Unspecified reactive species

Asvin K K Lakkaraju,Silvia Sorce,Assunta Senatore,Mario Nuvolone,Jingjing Guo,Petra Schwarz,Rita Moos,Pawel Pelczar,Adriano Aguzzi

Frontiers in pharmacology 13:807330 PubMed35185566

2022

Caffeine Functions by Inhibiting Dorsal and Ventral Hippocampal Adenosine 2A Receptors to Modulate Memory and Anxiety, Respectively.

Applications

Unspecified application

Species

Unspecified reactive species

Yawei Xu,Yalei Ning,Yan Zhao,Yan Peng,Fen Luo,Yuanguo Zhou,Ping Li
View all publications
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Product promise

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