Anti-SNAP25 antibody [EP3274]

9 product images

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  Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

  All lanes: Western blot - Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution

  Lane 1: Fetal brain lysate at 10 µg

  Lane 2: HeLa cell lysate at 10 µg

  Lane 3: SH-SY5Y cell lysate at 10 µg

  Lane 4: U87-MG cell lysate at 10 µg

  Lane 5: Fetal hippocampus lysate at 10 µg

  Lane 6: Fetal cerebellum lysate at 10 µg

  Lane 7: Neuro-2a cell lysate at 10 µg

  Predicted band size: 23 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

  ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.
  

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

  Blocking/Diluting buffer and concentration: 5% NFDM /TBST

  All lanes: Western blot - Anti-SNAP25 antibody [EP3274] (ab108990) at 1/2000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Lane 3: Mouse kidney tissue lysate at 20 µg

  Lane 4: Mouse spleen tissue lysate at 20 µg

  Lane 5: Rat brain tissue lysate at 20 µg

  Lane 6: Rat heart tissue lysate at 20 µg

  Lane 7: Rat kidney tissue lysate at 20 µg

  Lane 8: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

  Predicted band size: 23 kDa

  Observed band size: 25 kDa

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  Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

  False colour image of Western blot: Anti-SNAP25 antibody [EP3274] staining at 1/1000 dilution, shown in green; loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab108990 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line Human SNAP25 knockout SH-SY5Y cell line ab280041 (knockout cell lysate Human SNAP25 knockout SH-SY5Y cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

  All lanes: Western blot - Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution

  Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg

  Lane 2: SNAP25 knockout SH-SY5Y cell lysate at 20 µg

  Lane 2: Western blot - Human SNAP25 knockout SH-SY5Y cell line (Human SNAP25 knockout SH-SY5Y cell line ab280041)

  Lane 3: Neuro-2a cell lysate at 20 µg

  Lane 4: K562 cell lysate at 20 µg

  Performed under reducing conditions.

  Predicted band size: 23 kDa

  Observed band size: 27 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

  ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

  ab108990 showing negative staining in human Normal colon tissue.
  
  

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

  ab108990 showing negative staining in human Normal spleen tissue.
  
  

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (ab108990)

  ab108990 showing negative staining in human Skeletal muscle tissue.
  
  

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-SNAP25 antibody [EP3274] (ab108990)

  SNAP25 western blot using anti-SNAP25 antibody [EP3274] ab108990. Publication image and figure legend from Almandoz-Gil, L., Persson, E., et al., 2018, Front Neurol, PubMed 29623065.

  
  

  ab108990 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108990 please see the product overview.

  Representative images of immunofluorescence staining of non-tg cortical primary neurons (12 DIV) using antibodies against alpha-synuclein (αSyn) [mAb1338, red, (A)], VAMP-2 [(green), (B)], SNAP-25 [(green), (C)], and syntaxin-1 [(green), (D)]. DAPI in blue. Scale bar 50 µm.