anti-SNAP25 antibody [EP3274] ab226046 is a carrier free rabbit monoclonal antibody that is used in SNAP25 western blotting and IHC. Suitable for human, mouse and rat samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Specificity confirmed with SNAP25 knockout cell line validation
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138757.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046)")
![Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--western-blot-img168965.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98547.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98534.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (AB226046)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Tested |
| Rat | Expected | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
2 products for Alternative Version
Target data
Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Alternative names
Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP, SNAP, SNAP25
Recommended products
anti-SNAP25 antibody [EP3274] ab226046 is a carrier free rabbit monoclonal antibody that is used in SNAP25 western blotting and IHC. Suitable for human, mouse and rat samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Specificity confirmed with SNAP25 knockout cell line validation
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP3274
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226046 is the carrier-free version of Anti-SNAP25 antibody [EP3274] ab108990.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
- Biological function summary
SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.
- Pathways
One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.
- Associated diseases and disorders
SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
Anti-SNAP25 antibody [EP3274] ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAP25 antibody [EP3274] ab108990).
![Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--western-blot-img168965.jpg "Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
False colour image of Western blot: Anti-SNAP25 antibody [EP3274] staining at 1/1000 dilution, shown in green; loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, Anti-SNAP25 antibody [EP3274] ab108990 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line Human SNAP25 knockout SH-SY5Y cell line ab280041 (knockout cell lysate Human SNAP25 knockout SH-SY5Y cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-SNAP25 antibody [EP3274] (Anti-SNAP25 antibody [EP3274] ab108990) at 1/1000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg
Lane 2: SNAP25 knockout SH-SY5Y cell lysate at 20 µg
Lane 3: Neuro-2a cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139483.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
Anti-SNAP25 antibody [EP3274] ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAP25 antibody [EP3274] ab108990).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98547.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
Anti-SNAP25 antibody [EP3274] ab108990 showing negative staining in Normal colon tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAP25 antibody [EP3274] ab108990).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98534.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
Anti-SNAP25 antibody [EP3274] ab108990 showing negative staining in Normal spleen tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAP25 antibody [EP3274] ab108990).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114605.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
Anti-SNAP25 antibody [EP3274] ab108990 showing negative staining in Skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SNAP25 antibody [EP3274] ab108990).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-ep3274-bsa-and-azide-free-ab226046--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114605.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
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