Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting SNAP25 in ELISA. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Images
![Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (AB252660), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-epr20249-110-bsa-and-azide-free-capture-ab252660--sandwich-elisa-img52783.png/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (AB252660)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Alternative names
SNAP, SNAP25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP
Recommended products
Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting SNAP25 in ELISA. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20249-110
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human samples.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
- Biological function summary
SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.
- Pathways
One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.
- Associated diseases and disorders
SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660), expandable thumbnail](https://content.abcam.com/products/images/snap25-antibody-epr20249-110-bsa-and-azide-free-capture-ab252660--sandwich-elisa-img52783.png "Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660)")
Sandwich ELISA - Anti-SNAP25 antibody [EPR20249-110] - BSA and Azide free (Capture) (ab252660)
Representative standard curve from corresponding SimpleStep ELISA® Kit (SNAP25 ELISA Kit ab256394).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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