AB169871

Anti-SNAP25 (phospho S187) antibody

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(3 Publications)

Rabbit Polyclonal SNAP25 phospho S187 antibody. Suitable for WB and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Rat Snap25 phospho S187.

View Alternative Names

Snap, Snap25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP

1 Images

Western blot - Anti-SNAP25 (phospho S187) antibody (AB169871)

WB

Unknown

Western blot - Anti-SNAP25 (phospho S187) antibody (AB169871)

All lanes:

Western blot - Anti-SNAP25 (phospho S187) antibody (ab169871) at 1/1000 dilution

Lane 1:

Rat hippocampal lysate

Lane 2:

Rat hippocampal lysate treated with Lambda phosphatase (400 units/100 μl lysate for 30 min)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

Immunogen

Synthetic Peptide within Rat Snap25 phospho S187. The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab169871 is specific for SNAP25 protein phosphorylated at Ser187 in Western blots. Immunolabeling is completely blocked by blocked λ-Ptase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.

Biological function summary

SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.

Pathways

One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.

SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed : 8103915, PubMed : 8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (PubMed : 12403834).

See full target information Snap25 phospho S187

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 16:905403 PubMed35860293

2022

Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

ShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin Li

Experimental physiology 106:2235-2247 PubMed34605091

2021

Increased myofilament calcium sensitivity is associated with decreased cardiac troponin I phosphorylation in the diabetic rat heart.

Applications

Unspecified application

Species

Unspecified reactive species

Angela C Greenman,Gary M Diffee,Amelia S Power,Gerard T Wilkins,Olivia M S Gold,Jeffrey R Erickson,James C Baldi

Addiction biology 23:1067-1078 PubMed28884870

2017

Phosphorylated SNAP25 in the CA1 regulates morphine-associated contextual memory retrieval via increasing GluN2B-NMDAR surface localization.

Applications

Unspecified application

Species

Unspecified reactive species

Xinjuan Wang,Yan Liu,Meng Jia,Xiaowei Sun,Na Wang,Yijing Li,Cailian Cui

View all publications

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Product promise

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