Rabbit Polyclonal SNAP25 phospho S187 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Snap25 phospho S187.
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- Frontiers in neuroscience 16:9054032022Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuJuan Fan,Li Li,LiRong Liu,He Li,XiaoHui Xian,WenBin LiPubMed 35860293
- Addiction biology 23:1067-10782017Phosphorylated SNAP25 in the CA1 regulates morphine-associated contextual memory retrieval via increasing GluN2B-NMDAR surface localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinjuan Wang,Yan Liu,Meng Jia,Xiaowei Sun,Na Wang,Yijing Li,Cailian CuiPubMed 28884870
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Snap25 phospho S187. The exact immunogen used to generate this antibody is proprietary information. Database link P60881
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Guinea pig | Predicted |
| Primates | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Guinea pig, Human, Primates, Zebrafish | Dilution info - | Notes - |
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Target data
Function
t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed:8103915, PubMed:8243676). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells (PubMed:12403834).
Alternative names
Snap, Snap25, Synaptosomal-associated protein 25, SNAP-25, Super protein, Synaptosomal-associated 25 kDa protein, SUP
Recommended products
Rabbit Polyclonal SNAP25 phospho S187 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Snap25 phospho S187.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Snap25 phospho S187. The exact immunogen used to generate this antibody is proprietary information. Database link P60881
- Purification technique
- Affinity purification Immunogen
- Specificity
ab169871 is specific for SNAP25 protein phosphorylated at Ser187 in Western blots. Immunolabeling is completely blocked by blocked λ-Ptase.
- Concentration
- Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
- Biological function summary
SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.
- Pathways
One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.
- Associated diseases and disorders
SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-SNAP25 (phospho S187) antibody (ab169871)
All lanes: Western blot - Anti-SNAP25 (phospho S187) antibody (ab169871) at 1/1000 dilution
Lane 1: Rat hippocampal lysate
Lane 2: Rat hippocampal lysate treated with Lambda phosphatase (400 units/100 μl lysate for 30 min)
Predicted band size: 23 kDa
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