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Rabbit Recombinant Monoclonal SNAP29 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (AB240248), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (AB240248), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (AB240248), expandable thumbnail
  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (AB240248), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.

Alternative names

Synaptosomal-associated protein 29, SNAP-29, Soluble 29 kDa NSF attachment protein, Vesicle-membrane fusion protein SNAP-29, SNAP29

Recommended products

Rabbit Recombinant Monoclonal SNAP29 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR9198(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240248 is the carrier-free version of Anti-SNAP29 antibody [EPR9198(2)] ab181151.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SNAP29 known also as synaptosomal-associated protein 29 functions mainly in membrane fusion. It weighs about 29 kDa. This protein plays a significant role in intracellular membrane trafficking guiding vesicle fusion events. It is expressed in many tissues with high occurrence in neuronal and non-neuronal cells. SNAP29 contains coiled-coil domains that facilitate its role in binding with SNARE complexes which assist in the docking and fusion of vesicles with target membranes.

Biological function summary

RNA encoding this protein facilitates vesicle-mediated transport on the cellular level especially under conditions requiring rapid membrane addition or reshaping. SNAP29 acts alongside other SNARE proteins like syntaxin and VAMP in the SNARE complex to modulate membrane interactions. The protein eases the process of cytoplasmic content exchange between organelles. As part of these complexes SNAP29 maintains cellular homeostasis by contributing to membrane dynamics and trafficking processes.

Pathways

Vesicular transport in the cell highly depends on SNAP29 especially in exocytosis and endocytosis pathways. Alongside other SNARE proteins SNAP29 fits into the vesicle docking and fusion pathways ensuring efficient membrane recycling and cargo delivery. It interacts with several proteins in the process including syntaxin 1 and VAMP2 to facilitate these important functions within these pathways.

Associated diseases and disorders

Impairments or mutations in the SNAP29 gene link to conditions like CEDNIK syndrome and other neurodevelopmental disorders. CEDNIK syndrome arises from disrupted membrane trafficking and organelle biogenesis due to SNAP29 dysfunction. In these cases a relationship with other proteins in the disease pathways such as STXBP1 can also alter disease progression and severity influencing neuronal communication and stability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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