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AB138500

Anti-SNAP29 antibody [EPR9199]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

5

(4 Reviews)

|

(22 Publications)

Rabbit Recombinant Monoclonal SNAP29 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 22 publications.

View Alternative Names

Synaptosomal-associated protein 29, SNAP-29, Soluble 29 kDa NSF attachment protein, Vesicle-membrane fusion protein SNAP-29, SNAP29

6 Images
Immunoprecipitation - Anti-SNAP29 antibody [EPR9199] (AB138500)
  • IP

Unknown

Immunoprecipitation - Anti-SNAP29 antibody [EPR9199] (AB138500)

ab138500 (purified) at 1/20 immunoprecipitating SNAP29 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-SNAP29 antibody [EPR9199] (ab138500)

Predicted band size: 21 kDa,29 kDa

Observed band size: 21 kDa,22 kDa

false

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)
  • WB

Lab

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)

Lanes 1-3 : Merged signal (red and green). Green - ab138500 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab138500 Anti-SNAP29 antibody [EPR9199] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab138500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SNAP29 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SNAP29 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-snap29-knockout-hela-cell-line-ab265289'>ab265289</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)
  • WB

Lab

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HEK293 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)
  • WB

Lab

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)
  • WB

Unknown

Western blot - Anti-SNAP29 antibody [EPR9199] (AB138500)

All lanes:

Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

OI-RD Scanning - Anti-SNAP29 antibody [EPR9199] (AB138500)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SNAP29 antibody [EPR9199] (AB138500)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SNAP29 antibody [EPR9199] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9199

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAP29 known also as synaptosomal-associated protein 29 functions mainly in membrane fusion. It weighs about 29 kDa. This protein plays a significant role in intracellular membrane trafficking guiding vesicle fusion events. It is expressed in many tissues with high occurrence in neuronal and non-neuronal cells. SNAP29 contains coiled-coil domains that facilitate its role in binding with SNARE complexes which assist in the docking and fusion of vesicles with target membranes.
Biological function summary

RNA encoding this protein facilitates vesicle-mediated transport on the cellular level especially under conditions requiring rapid membrane addition or reshaping. SNAP29 acts alongside other SNARE proteins like syntaxin and VAMP in the SNARE complex to modulate membrane interactions. The protein eases the process of cytoplasmic content exchange between organelles. As part of these complexes SNAP29 maintains cellular homeostasis by contributing to membrane dynamics and trafficking processes.

Pathways

Vesicular transport in the cell highly depends on SNAP29 especially in exocytosis and endocytosis pathways. Alongside other SNARE proteins SNAP29 fits into the vesicle docking and fusion pathways ensuring efficient membrane recycling and cargo delivery. It interacts with several proteins in the process including syntaxin 1 and VAMP2 to facilitate these important functions within these pathways.

Impairments or mutations in the SNAP29 gene link to conditions like CEDNIK syndrome and other neurodevelopmental disorders. CEDNIK syndrome arises from disrupted membrane trafficking and organelle biogenesis due to SNAP29 dysfunction. In these cases a relationship with other proteins in the disease pathways such as STXBP1 can also alter disease progression and severity influencing neuronal communication and stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.
See full target information Synaptosomal-associated protein 29
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 21:100700 PubMed37455821

2023

Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of to alleviate tissue infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Peilin,Peng Ying,Wang Renyuan,Li Zhuoxuan,Yang Zhenwu,Zhao Mai,Song Jianguo,Zhang Hao,Yin Gang,Lin Lin,Lin Haodong

Cellular and molecular life sciences : CMLS 80:177 PubMed37285022

2023

siRNA screening reveals that SNAP29 contributes to exosome release.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Pettersen Hessvik,Krizia Sagini,Silvana Romero,Manuel Ramirez-Garrastacho,Marta Rodriguez,Astrid Elisabeth V Tutturen,Audun Kvalvaag,Espen Stang,Andreas Brech,Kirsten Sandvig,Alicia Llorente

Autophagy 19:112-125 PubMed35446171

2022

Starvation after infection restricts enterovirus D68 replication.

Applications

Unspecified application

Species

Unspecified reactive species

Alagie Jassey,Michael A Wagner,Ganna Galitska,Bimal Paudel,Katelyn Miller,William T Jackson

The Journal of cell biology 221: PubMed35446347

2022

Secretory autophagy maintains proteostasis upon lysosome inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Tina A Solvik,Tan A Nguyen,Yu-Hsiu Tony Lin,Timothy Marsh,Eric J Huang,Arun P Wiita,Jayanta Debnath,Andrew M Leidal

Developmental cell 56:427-442.e5 PubMed33422265

2021

ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyan Miao,Hongyu Zhao,Yan Li,Mingming Ji,Yong Chen,Yi Shi,Yuhai Bi,Peihui Wang,Hong Zhang

The Journal of biological chemistry 296:100268 PubMed33837726

2021

SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.

Applications

Unspecified application

Species

Unspecified reactive species

Rodolfo A Cardenas,Ricardo Gonzalez,Elizabeth Sanchez,Marco A Ramos,Eduardo I Cardenas,Alejandro I Rodarte,Roberto J Alcazar-Felix,Alejandro Isaza,Alan R Burns,Ruth Heidelberger,Roberto Adachi

Autophagy 17:3068-3081 PubMed33213278

2020

Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Subhankar Dolai,Toshimasa Takahashi,Tairan Qin,Tao Liang,Li Xie,Fei Kang,Yi-Fan Miao,Huanli Xie,Youhou Kang,Justin Manuel,Erin Winter,Paul A Roche,Mark S Cattral,Herbert Y Gaisano

PLoS pathogens 16:e1009017 PubMed33052966

2020

A non-canonical role for the autophagy machinery in anti-retroviral signaling mediated by TRIM5α.

Applications

Unspecified application

Species

Unspecified reactive species

Bhaskar Saha,Devon Chisholm,Alison M Kell,Michael A Mandell

Human genetics 138:1409-1417 PubMed31748968

2019

Compound heterozygous mutations in SNAP29 is associated with Pelizaeus-Merzbacher-like disorder (PMLD).

Applications

Unspecified application

Species

Unspecified reactive species

Lorida Llaci,Keri Ramsey,Newell Belnap,Ana M Claasen,Chris D Balak,Szabolcs Szelinger,Wayne M Jepsen,Ashley L Siniard,Ryan Richholt,Tyler Izat,Marcus Naymik,Matt De Both,Ignazio S Piras,David W Craig,Matthew J Huentelman,Vinodh Narayanan,Isabelle Schrauwen,Sampathkumar Rangasamy

PloS one 14:e0223254 PubMed31714914

2019

SNARE proteins rescue impaired autophagic flux in Down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanos Aivazidis,Abhilasha Jain,Abhishek K Rauniyar,Colin C Anderson,John O Marentette,David J Orlicky,Kristofer S Fritz,Peter S Harris,David Siegel,Kenneth N Maclean,James R Roede
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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