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AB251226

Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free

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Rabbit Recombinant Monoclonal SNAPC1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

SNAP43, SNAPC1, snRNA-activating protein complex subunit 1, SNAPc subunit 1, Proximal sequence element-binding transcription factor subunit gamma, Small nuclear RNA-activating complex polypeptide 1, snRNA-activating protein complex 43 kDa subunit, PSE-binding factor subunit gamma, PTF subunit gamma, SNAPc 43 kDa subunit

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)

This data was developed using ab197015, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling SNAPC1 with ab197015 at 1/250 dilution, followed by Alexa Fluor®488 Goat Anti-Rabbit IgG H&L (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab197015 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Flow Cytometry (Intracellular) - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)

This data was developed using ab197015, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling SNAPC1 with ab197015 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)
  • WB

Supplier Data

Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)

This data was developed using ab197015, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNAPC1 antibody [EPR16466] (<a href='/en-us/products/primary-antibodies/snapc1-antibody-epr16466-ab197015'>ab197015</a>) at 1/5000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)
  • WB

Supplier Data

Western blot - Anti-SNAPC1 antibody [EPR16466] - BSA and Azide free (AB251226)

This data was developed using ab197015, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNAPC1 antibody [EPR16466] (<a href='/en-us/products/primary-antibodies/snapc1-antibody-epr16466-ab197015'>ab197015</a>) at 1/5000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16466

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251226 is the carrier-free version of ab197015.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNAPC1 also known as small nuclear RNA activating complex polypeptide 1 is a component of the snRNA-activating protein complex. It has a mass of about 43 kDa. SNAPC1 plays an important role in the transcription initiation of small nuclear RNA (snRNA) genes. It functions through interaction with other components of the SNAPc complex. Researchers observe its expression in various tissues but it appears particularly active in cells with high transcriptional demand.
Biological function summary

The role of SNAPC1 lies in the assembly and regulation of the SNAPc complex. This complex functions primarily in the transcription of snRNA essential for mRNA processing and gene expression regulation. By participating in the formation of the snRNA-activating protein complex SNAPC1 facilitates the correct transcription and processing of snRNA a critical component in the spliceosomal machinery. It achieves this by forming stable interactions with other SNAPc components including SNAPC2 and SNAPC3 contributing to pre-mRNA splicing.

Pathways

SNAPC1 integrates into two major cellular pathways: transcription initiation and RNA splicing. It closely interacts with elements such as RNA polymerase II and SNAPC4 within these pathways. By securing a stable interaction with these elements SNAPC1 supports efficient transcription of snRNA and its subsequent processing. The proper function of SNAPC1 and related proteins ensures the accuracy of mRNA splicing impacting overall gene expression and cellular function.

Defects or dysregulations involving SNAPC1 can link to disorders like Spinal Muscular Atrophy (SMA) and systemic lupus erythematosus (SLE). In SMA disruptions in snRNA processing lead to defects in the creation of essential proteins needed for motor neuron survival wherein SNAPC1 may hold a supporting role. In SLE abnormal snRNA processing can contribute to the production of autoantibodies linking SNAPC1 to the autoimmune pathway. SNAPC1's functional interactions with proteins like SMN (Survival of Motor Neuron) protein in SMA further illustrate its involvement in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.
See full target information SNAPC1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com