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Rabbit Polyclonal SNAPIN antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SNARE-associated protein Snapin aa 1 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAPIN antibody (AB254583), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAPIN antibody (AB254583), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SNARE-associated protein Snapin aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95295

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells (PubMed:17182842, PubMed:18167355). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PubMed:25898167).

Alternative names

Recommended products

Rabbit Polyclonal SNAPIN antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SNARE-associated protein Snapin aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SNARE-associated protein Snapin aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95295
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SNAPIN also known as SNAP25-interacting protein has an approximate mass of 15 kDa and functions mechanically by interacting with the SNARE complex an important structure in vesicular trafficking. It binds directly to the SNAP25 protein facilitating synaptic vesicle docking and neurotransmitter release at the synapse. Expression of SNAPIN is widespread throughout neuronal tissues and other cell types indicating its role in cellular communication processes.

Biological function summary

SNAPIN enhances the efficiency of synaptic transmission and vesicle fusion processes. It integrates into the SNARE complex where it stabilizes this multi-protein assembly essential for neurotransmitter release. By bridging interactions between SNAP25 and synaptic vesicles SNAPIN helps bridge essential synapse communication modulating the synaptic transmission needed for effective neuron signaling.

Pathways

SNAPIN participates in the synaptic vesicle cycle and neurotransmitter release pathways. It works closely in tandem with proteins such as Synaptotagmin and Synaptobrevin which are vital players in the SNARE complex responsible for membrane fusion. By modulating neurotransmitter release SNAPIN contributes to synaptic plasticity and response to neurological stimuli linking it to broader neurological signaling pathways.

Associated diseases and disorders

Dysfunction of SNAPIN can relate to neurodegenerative diseases including Alzheimer's disease due to its role in neurotransmitter release and synaptic function. Misregulation of SNAPIN function might also implicate it in psychiatric disorders such as schizophrenia where synaptic communication deficits are present. Additionally its involvement with SNAP25 in the SNARE complex associates it with these diseases and potential disruptions in SNARE-associated pathways and synaptic communication.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAPIN antibody (ab254583), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAPIN antibody (ab254583)

    Paraffin-embedded human testis tissue stained for SNAPIN using ab254583 at 1/50 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAPIN antibody (ab254583), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SNAPIN antibody (ab254583)

    PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling SNAPIN using ab254583 at 4µg/ml (green) in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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