AB225620

Anti-SND1 antibody

Name: Anti-SND1 antibody (ab225620) | Abcam
Brand: Abcam Limited
SKU: AB225620
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SND1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SND1 aa 100-150.

View Alternative Names

TDRD11, SND1, Staphylococcal nuclease domain-containing protein 1, 100 kDa coactivator, EBNA2 coactivator p100, Tudor domain-containing protein 11, p100 co-activator

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (AB225620)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (AB225620)

Formalin fixed, paraffin embedded human lung carcinoma tissue stained for SND1 using ab225620 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-SND1 antibody (AB225620)

SND1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225620 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225620 at 0.4 μg/ml.

Lane 1 : ab225620 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SND1 antibody (ab225620)

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-SND1 antibody (AB225620)

All lanes:

Western blot - Anti-SND1 antibody (ab225620) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 102 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human SND1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q7KZF4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab225620 was affinity purified using an epitope specific to SND1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Staphylococcal nuclease and Tudor domain-containing protein 1 (SND1) also known as Tudor-SN is a multifunctional protein with a molecular mass of approximately 100 kDa. SND1 acts as a coactivator in transcription and has nucleolytic activity tied to RNA processing and degradation. Researchers observe its wide expression in various tissues with higher levels in the liver brain and heart. Functionally significant SND1 binds to several molecules including double-stranded RNA which provides it with broad regulatory roles.

Biological function summary

SND1 engages in several cellular functions such as mRNA editing and splicing. It is a component of the RNA-induced silencing complex (RISC) which is essential in gene regulation through RNA interference. Within this complex SND1 interacts with other proteins and nucleic acids to contribute to post-transcriptional gene silencing. SND1 also influences transcriptional activation by interacting with transcription factors enhancing the expression of specific genes.

Pathways

SND1 plays roles in two important areas: RNA interference (RNAi) and lipid metabolism. In the RNAi pathway it facilitates gene silencing by being part of RISC collaborating with proteins such as Argonaute to mediate target mRNA degradation. In lipid metabolism SND1 interacts with proteins like sterol regulatory element-binding proteins (SREBPs) linking it to cholesterol homeostasis and fatty acid biosynthesis.

SND1 is linked to several cancer types including liver cancer and breast cancer. Its overexpression has been associated with tumor progression and poor prognosis in these conditions. SND1's interaction with oncogenic proteins such as Myc supports its role in cancer development. Additionally researchers have identified a connection between SND1 and Alzheimer's disease where it may influence neurodegenerative processes through interactions with amyloid precursor protein (APP) and other proteins involved in neuronal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endonuclease that mediates miRNA decay of both protein-free and AGO2-loaded miRNAs (PubMed : 18453631, PubMed : 28546213). As part of its function in miRNA decay, regulates mRNAs involved in G1-to-S phase transition (PubMed : 28546213). Functions as a bridging factor between STAT6 and the basal transcription factor (PubMed : 12234934). Plays a role in PIM1 regulation of MYB activity (PubMed : 9809063). Functions as a transcriptional coactivator for STAT5 (By similarity).. (Microbial infection) Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).. (Microbial infection) Promotes SARS-CoV-2 RNA synthesis by binding to negative-sense RNA and the viral protein nsp9.

See full target information SND1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 30:963689720983786 PubMed33588586

2021

Circ SMARCA5 Inhibited Tumor Metastasis by Interacting with SND1 and Downregulating the YWHAB Gene in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zhang,Qing Zhang,Ke Zhang,Fang Wang,Xiaogai Qiao,Jinquan Cui

Analytical cellular pathology (Amsterdam) 2019:1575031 PubMed30881868

2019

ILF2 Directly Binds and Stabilizes CREB to Stimulate Malignant Phenotypes of Liver Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Du,Yun Le,Fenyong Sun,Kai Li,Yanfeng Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SND1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (AB225620)

Immunoprecipitation - Anti-SND1 antibody (AB225620)

Western blot - Anti-SND1 antibody (AB225620)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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