Rabbit Polyclonal SND1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SND1 aa 100-150.
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Publications
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- Cell transplantation 30:9636897209837862021Circ SMARCA5 Inhibited Tumor Metastasis by Interacting with SND1 and Downregulating the YWHAB Gene in Cervical Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Zhang,Qing Zhang,Ke Zhang,Fang Wang,Xiaogai Qiao,Jinquan CuiPubMed 33588586
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SND1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q7KZF4
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Endonuclease that mediates miRNA decay of both protein-free and AGO2-loaded miRNAs (PubMed:18453631, PubMed:28546213). As part of its function in miRNA decay, regulates mRNAs involved in G1-to-S phase transition (PubMed:28546213). Functions as a bridging factor between STAT6 and the basal transcription factor (PubMed:12234934). Plays a role in PIM1 regulation of MYB activity (PubMed:9809063). Functions as a transcriptional coactivator for STAT5 (By similarity). (Microbial infection) Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2). (Microbial infection) Promotes SARS-CoV-2 RNA synthesis by binding to negative-sense RNA and the viral protein nsp9.
Alternative names
TDRD11, SND1, Staphylococcal nuclease domain-containing protein 1, 100 kDa coactivator, EBNA2 coactivator p100, Tudor domain-containing protein 11, p100 co-activator
Recommended products
Rabbit Polyclonal SND1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SND1 aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SND1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q7KZF4
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab225620 was affinity purified using an epitope specific to SND1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Staphylococcal nuclease and Tudor domain-containing protein 1 (SND1) also known as Tudor-SN is a multifunctional protein with a molecular mass of approximately 100 kDa. SND1 acts as a coactivator in transcription and has nucleolytic activity tied to RNA processing and degradation. Researchers observe its wide expression in various tissues with higher levels in the liver brain and heart. Functionally significant SND1 binds to several molecules including double-stranded RNA which provides it with broad regulatory roles.
- Biological function summary
SND1 engages in several cellular functions such as mRNA editing and splicing. It is a component of the RNA-induced silencing complex (RISC) which is essential in gene regulation through RNA interference. Within this complex SND1 interacts with other proteins and nucleic acids to contribute to post-transcriptional gene silencing. SND1 also influences transcriptional activation by interacting with transcription factors enhancing the expression of specific genes.
- Pathways
SND1 plays roles in two important areas: RNA interference (RNAi) and lipid metabolism. In the RNAi pathway it facilitates gene silencing by being part of RISC collaborating with proteins such as Argonaute to mediate target mRNA degradation. In lipid metabolism SND1 interacts with proteins like sterol regulatory element-binding proteins (SREBPs) linking it to cholesterol homeostasis and fatty acid biosynthesis.
- Associated diseases and disorders
SND1 is linked to several cancer types including liver cancer and breast cancer. Its overexpression has been associated with tumor progression and poor prognosis in these conditions. SND1's interaction with oncogenic proteins such as Myc supports its role in cancer development. Additionally researchers have identified a connection between SND1 and Alzheimer's disease where it may influence neurodegenerative processes through interactions with amyloid precursor protein (APP) and other proteins involved in neuronal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-SND1 antibody (ab225620)
All lanes: Western blot - Anti-SND1 antibody (ab225620) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab225620)
Formalin fixed, paraffin embedded human lung carcinoma tissue stained for SND1 using ab225620 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Immunoprecipitation - Anti-SND1 antibody (ab225620)
SND1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225620 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225620 at 0.4 μg/ml.
Lane 1: ab225620 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-SND1 antibody (ab225620)
Predicted band size: 102 kDa
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