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Rabbit Recombinant Monoclonal SND1 antibody. Suitable for I-ELISA, ICC/IF, IHC-P, WB and reacts with Recombinant fragment - Human, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody [EPR28903-10] (AB322051), expandable thumbnail
  • Western blot - Anti-SND1 antibody [EPR28903-10] (AB322051), expandable thumbnail
  • Western blot - Anti-SND1 antibody [EPR28903-10] (AB322051), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (AB322051), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (AB322051), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAICC/IFIHC-PWBIP
Human
Expected
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1000 ng/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Endonuclease that mediates miRNA decay of both protein-free and AGO2-loaded miRNAs (PubMed:18453631, PubMed:28546213). As part of its function in miRNA decay, regulates mRNAs involved in G1-to-S phase transition (PubMed:28546213). Functions as a bridging factor between STAT6 and the basal transcription factor (PubMed:12234934). Plays a role in PIM1 regulation of MYB activity (PubMed:9809063). Functions as a transcriptional coactivator for STAT5 (By similarity). (Microbial infection) Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2). (Microbial infection) Promotes SARS-CoV-2 RNA synthesis by binding to negative-sense RNA and the viral protein nsp9.

Alternative names

TDRD11, SND1, Staphylococcal nuclease domain-containing protein 1, 100 kDa coactivator, EBNA2 coactivator p100, Tudor domain-containing protein 11, p100 co-activator

Rabbit Recombinant Monoclonal SND1 antibody. Suitable for I-ELISA, ICC/IF, IHC-P, WB and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28903-10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Staphylococcal nuclease and Tudor domain-containing protein 1 (SND1) also known as Tudor-SN is a multifunctional protein with a molecular mass of approximately 100 kDa. SND1 acts as a coactivator in transcription and has nucleolytic activity tied to RNA processing and degradation. Researchers observe its wide expression in various tissues with higher levels in the liver brain and heart. Functionally significant SND1 binds to several molecules including double-stranded RNA which provides it with broad regulatory roles.

Biological function summary

SND1 engages in several cellular functions such as mRNA editing and splicing. It is a component of the RNA-induced silencing complex (RISC) which is essential in gene regulation through RNA interference. Within this complex SND1 interacts with other proteins and nucleic acids to contribute to post-transcriptional gene silencing. SND1 also influences transcriptional activation by interacting with transcription factors enhancing the expression of specific genes.

Pathways

SND1 plays roles in two important areas: RNA interference (RNAi) and lipid metabolism. In the RNAi pathway it facilitates gene silencing by being part of RISC collaborating with proteins such as Argonaute to mediate target mRNA degradation. In lipid metabolism SND1 interacts with proteins like sterol regulatory element-binding proteins (SREBPs) linking it to cholesterol homeostasis and fatty acid biosynthesis.

Associated diseases and disorders

SND1 is linked to several cancer types including liver cancer and breast cancer. Its overexpression has been associated with tumor progression and poor prognosis in these conditions. SND1's interaction with oncogenic proteins such as Myc supports its role in cancer development. Additionally researchers have identified a connection between SND1 and Alzheimer's disease where it may influence neurodegenerative processes through interactions with amyloid precursor protein (APP) and other proteins involved in neuronal health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SND1 with ab322051 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 dilution (Green).

    Confocal image showing cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 dilution.

  • Western blot - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Western blot - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SND1 antibody [EPR28903-10] (ab322051) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting SND1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 101 kDa, 36 kDa

    Exposure time: 10s

  • Western blot - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Western blot - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SND1 antibody [EPR28903-10] (ab322051) at 1/1000 dilution

    Lane 1: A549 (human lung carcinoma epithelial cell) whole lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 101 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SND1 with ab322051 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: Weak staining in human skeletal muscle.

    The section was incubated with ab322051 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SND1 with ab322051 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human colon carcinoma.

    The section was incubated with ab322051 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling SND1 with ab322051 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human hepatocellular carcinoma.

    The section was incubated with ab322051 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SND1 with ab322051 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human colon.

    The section was incubated with ab322051 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Indirect ELISA - Anti-SND1 antibody [EPR28903-10] (ab322051), expandable thumbnail

    Indirect ELISA - Anti-SND1 antibody [EPR28903-10] (ab322051)

    Indirect ELISA analysis of ab322051 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Human SND1.

    Antigen concentration: 1000 ng/ml

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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