Anti-SND1 antibody [EPR28903-23] - BSA and Azide free
- RabMAb
- Recombinant
- BOND RX™ Validated
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Rabbit Recombinant Monoclonal SND1 antibody. Carrier free. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
View Alternative Names
TDRD11, SND1, Staphylococcal nuclease domain-containing protein 1, 100 kDa coactivator, EBNA2 coactivator p100, Tudor domain-containing protein 11, p100 co-activator
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue : No staining in human skeletal muscle.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human cerebrum (PMID : 30214229).
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human colon carcinoma (PMID : 25965817).
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human colon.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human hepatocellular carcinoma (PMID : 30321081).
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue : No staining in mouse skeletal muscle.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse cerebrum.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse colon.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tumor tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse liver tumor.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- WB
Supplier Data
Western blot - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody was tested on frozen and fresh NIH/3T3 whole cell lysates and it was concluded that SND1 is the easy target for degradation.
To minimize protein degradation, cells (lane6-9) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
The band(around 60 kd) beneath the target band is likely to be degraded target fragment.
The band be lower MW band around 50 kDa is supposed to be non-specific binding.
All lanes:
Western blot - Anti-SND1 antibody [EPR28903-23] (<a href='/en-us/products/primary-antibodies/snd1-antibody-epr28903-23-ab322053'>ab322053</a>) at 1/1000 dilution
Lane 1:
A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 3:
HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
MEF (mouse embryo fibroblast) whole cell lysate at 20 µg
Lanes 5 and 8:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 6:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 7:
293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 9:
PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 101 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The identity of the lower MW band around 50 kDa is supposed to be non-specific binding.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-SND1 antibody [EPR28903-23] (<a href='/en-us/products/primary-antibodies/snd1-antibody-epr28903-23-ab322053'>ab322053</a>) at 1/1000 dilution
Lane 1:
HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2:
HeLa transfected with siRNA specifically targeting SND1 whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 101 kDa,36 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Low expression : skeletal muscle
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-SND1 antibody [EPR28903-23] (<a href='/en-us/products/primary-antibodies/snd1-antibody-epr28903-23-ab322053'>ab322053</a>) at 1/1000 dilution
Lane 1:
Mouse liver tissue lysate at 20 µg
Lane 2:
Mouse pancreas tissue lysate at 20 µg
Lane 3:
Mouse skeletal muscle tissue lysate at 20 µg
Lane 4:
Rat liver tissue lysate at 20 µg
Lane 5:
Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 101 kDa,36 kDa
false
Exposure time: 6s
- I-ELISA
Supplier Data
Indirect ELISA - Anti-SND1 antibody [EPR28903-23] - BSA and Azide free (AB322054)
This data was developed using ab322053, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of ab322053 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.
Antigen : Human SND1.
Antigen concentration : 1000 ng/ml
Related conjugates and formulations (1)
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Anti-SND1 antibody [EPR28903-23]
Reactivity data
Product details
ab322054 is the carrier-free version of ab322053.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SND1 engages in several cellular functions such as mRNA editing and splicing. It is a component of the RNA-induced silencing complex (RISC) which is essential in gene regulation through RNA interference. Within this complex SND1 interacts with other proteins and nucleic acids to contribute to post-transcriptional gene silencing. SND1 also influences transcriptional activation by interacting with transcription factors enhancing the expression of specific genes.
Pathways
SND1 plays roles in two important areas: RNA interference (RNAi) and lipid metabolism. In the RNAi pathway it facilitates gene silencing by being part of RISC collaborating with proteins such as Argonaute to mediate target mRNA degradation. In lipid metabolism SND1 interacts with proteins like sterol regulatory element-binding proteins (SREBPs) linking it to cholesterol homeostasis and fatty acid biosynthesis.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com