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AB249700

Anti-SNF2L antibody [EPR12191] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SNF2L antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

SNF2L, SNF2L1, SMARCA1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A1, Global transcription activator SNF2L1, Nucleosome-remodeling factor subunit SNF2L, SNF2 related chromatin remodeling ATPase 1

4 Images
Flow Cytometry (Intracellular) - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)

This data was developed using ab172483, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling SNF2L with ab172483 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

Immunoprecipitation - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)
  • IP

Supplier Data

Immunoprecipitation - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-SNF2L antibody [EPR12191] (<a href='/en-us/products/primary-antibodies/snf2l-antibody-epr12191-ab172483'>ab172483</a>) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Lane 2:

RAW264.7 whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/snf2l-antibody-epr12191-ab172483'>ab172483</a> in RAW264.7 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 123 kDa

Observed band size: 123 kDa

true

Exposure time: 3min

Western blot - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)
  • WB

Supplier Data

Western blot - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)

This data was developed using ab172483, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNF2L antibody [EPR12191] (<a href='/en-us/products/primary-antibodies/snf2l-antibody-epr12191-ab172483'>ab172483</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

SK-OV-3 cell lysate at 10 µg

Predicted band size: 123 kDa

false

Western blot - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)
  • WB

Supplier Data

Western blot - Anti-SNF2L antibody [EPR12191] - BSA and Azide free (AB249700)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-SNF2L antibody [EPR12191] (<a href='/en-us/products/primary-antibodies/snf2l-antibody-epr12191-ab172483'>ab172483</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 123 kDa

Observed band size: 123 kDa

true

Exposure time: 81s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12191

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Flow Cyt (Intra) and IP results based on Human reactivity.

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Assay Kits

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249700 is the carrier-free version of ab172483.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNF2L also known as SMARCA1 is a protein that functions as a chromatin remodeler. The molecular weight of SNF2L approximately 136 kDa. SNF2L is expressed widely with a higher presence in the brain and testis. The protein plays an active role in the regulation of gene expression by altering chromatin structure through ATP-dependent mechanisms. SNF2L belongs to the ISWI family of chromatin remodelers which facilitates the process by sliding or restructuring nucleosomes on DNA.
Biological function summary

SNF2L acts as part of the NURF (Nucleosome Remodeling Factor) complex. This complex is involved in modifying chromatin to make DNA accessible for transcription machinery. In addition to its role in transcription regulation SNF2L contributes to DNA replication and repair processes. By affecting chromatin architecture SNF2L influences the cell cycle and various developmental pathways. SNF2L's action is essential for maintaining proper gene expression patterns necessary for normal cellular function.

Pathways

SNF2L contributes to the Wnt signaling and neuronal differentiation pathways. In these contexts SNF2L works with proteins such as β-catenin in Wnt signaling where its chromatin remodeling abilities facilitate the transcription of Wnt target genes. Additionally SNF2L interacts with neural transcription factors during neuronal differentiation guiding the chromatin remodeling efforts necessary for neurogenesis. These pathways illustrate SNF2L's importance in regulating genes particularly those involved in cell fate decisions.

SNF2L has been associated with neurodevelopmental disorders and cancer. Mutations or dysregulation of SNF2L can lead to intellectual disabilities and other brain-related anomalies highlighting its significance in cognitive function. In cancer anomalies in the expression or mutation of SNF2L can influence tumor progression potentially through pathways involving other tumor-related chromatin remodelers like BRCA1. As a result understanding SNF2L can offer insights into therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. ATPase that possesses intrinsic ATP-dependent chromatin-remodeling activity (PubMed : 14609955, PubMed : 15310751, PubMed : 15640247, PubMed : 28801535). ATPase activity is substrate-dependent, and is increased when nucleosomes are the substrate, but is also catalytically active when DNA alone is the substrate (PubMed : 14609955, PubMed : 15310751, PubMed : 15640247). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 14609955, PubMed : 15310751, PubMed : 15640247, PubMed : 28801535). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (PubMed : 28801535). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (PubMed : 28801535). The BAZ1A-, BAZ1B-, BAZ2A- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). Within the NURF-1 and CERF-1 ISWI chromatin remodeling complexes, nucleosomes are the preferred substrate for its ATPase activity (PubMed : 14609955, PubMed : 15640247). Within the NURF-1 ISWI chromatin-remodeling complex, binds to the promoters of En1 and En2 to positively regulate their expression and promote brain development (PubMed : 14609955). May promote neurite outgrowth (PubMed : 14609955). May be involved in the development of luteal cells (PubMed : 16740656). Facilitates nucleosome assembly during DNA replication, ensuring replication fork progression and genomic stability by preventing replication stress and nascent DNA gaps (PubMed : 39413208).. Isoform 2. Catalytically inactive when either DNA or nucleosomes are the substrate and does not possess chromatin-remodeling activity (PubMed : 15310751, PubMed : 28801535). Acts as a negative regulator of chromatin remodelers by generating inactive complexes (PubMed : 15310751).
See full target information SMARCA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

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