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Rabbit Recombinant Monoclonal SNF5/SMARCB1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

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Images

Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (AB250544), expandable thumbnail
  • Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (AB250544), expandable thumbnail
  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (AB250544), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

Alternative names

BAF47, INI1, SNF5L1, SMARCB1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, Integrase interactor 1 protein, SNF5 homolog, hSNF5

Recommended products

Rabbit Recombinant Monoclonal SNF5/SMARCB1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR12014
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250544 is the carrier-free version of Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SNF5/SMARCB1 also known as INI1 or BAF47 is a member of the SWI/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5/SMARCB1 facilitates transcriptional regulation and chromosomal stability.

Biological function summary

SNF5/SMARCB1 acts as an integral component of the SWI/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.

Pathways

The involvement of SNF5/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.

Associated diseases and disorders

SNF5/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5/SMARCB1 disrupts the SWI/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544), expandable thumbnail

    Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)

    This data was developed using Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] (Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976) at 1/5000 dilution

    Lane 1: 293 cell lysate at 20 µg

    Lane 2: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

  • Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544), expandable thumbnail

    Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)

    This data was developed using Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976, the same antibody clone in a different buffer formulation.Western blot analysis of HeLa cell lysate immunoprecipitated with Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976 at 1/50 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] (Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976)

    Predicted band size: 44 kDa

  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544), expandable thumbnail

    Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)

    This data was developed using Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] (Anti-SNF5/SMARCB1 antibody [EPR12014] ab181976) at 1/20000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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