Skip to main content

Rabbit Recombinant Monoclonal SNF5/SMARCB1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR20189] (AB222519), expandable thumbnail
  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (AB222519), expandable thumbnail
  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (AB222519), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (AB222519), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (AB222519), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

Alternative names

BAF47, INI1, SNF5L1, SMARCB1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, Integrase interactor 1 protein, SNF5 homolog, hSNF5

Recommended products

Rabbit Recombinant Monoclonal SNF5/SMARCB1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20189
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SNF5/SMARCB1 also known as INI1 or BAF47 is a member of the SWI/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5/SMARCB1 facilitates transcriptional regulation and chromosomal stability.

Biological function summary

SNF5/SMARCB1 acts as an integral component of the SWI/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.

Pathways

The involvement of SNF5/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.

Associated diseases and disorders

SNF5/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5/SMARCB1 disrupts the SWI/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    SNF5/SMARCB1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222519 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222519 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (input).
    Lane 2: ab222519 IP in HeLa whole cell lysate,
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab222519 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Predicted band size: 44 kDa

  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: G-401 (PMID: 19789351).

    All lanes: Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: G-401 (Human rhabdoid tumor kidney epithelial cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

    Exposure time: 10s

  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times : Lane 1-3: 2 seconds; Lanes 4-5/7: 15 seconds; Lane 6/8-9: 4 seconds; Lane 10: 1 minute.

    All lanes: Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

    Lane 4: Human fetal brain lysate at 10 µg

    Lane 5: Human fetal heart lysate at 10 µg

    Lane 6: Human fetal spleen lysate at 10 µg

    Lane 7: Mouse brain lysate at 10 µg

    Lane 8: Mouse spleen lysate at 10 µg

    Lane 9: Rat brain lysate at 10 µg

    Lane 10: Rat spleen lysate at 10 µg

    Secondary

    Lanes 1, 10, 2, 3, 7, 8 and 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 4 - 6: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Nuclear staining in human kidney is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Nuclear staining in mouse kidney is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Nuclear staining in rat kidney is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SNF5/SMARCB1 with ab222519 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    Negative control: G-401 cell line (PMID:19789351).

  • Flow Cytometry (Intracellular) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeablizedG-401 (Human rhabdoid tumor kidney epithelial cell line, Left) and HeLa (Human epithelial cell line from cervix adenocarcinoma, Right) cells labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: G-401 (PMID:19789351)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com