JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126734

Anti-SNF5/SMARCB1 antibody [EPR6966]

4

(3 Reviews)

|

(6 Publications)

Rabbit Recombinant Monoclonal SNF5/SMARCB1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

BAF47, INI1, SNF5L1, SMARCB1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, Integrase interactor 1 protein, SNF5 homolog, hSNF5

3 Images
Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (AB126734)
  • WB

Unknown

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (AB126734)

All lanes:

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 44 kDa

true

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (AB126734)
  • WB

AbReview42809****

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (AB126734)

All lanes:

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/2000 dilution

All lanes:

Zebrafish whole mount purified protein at 15 µg

Secondary

All lanes:

Undiluted HRP-conjugated goat anti-rabbit IgG polyclonal

Predicted band size: 44 kDa

Observed band size: 15 kDa,28 kDa,44 kDa

true

Exposure time: 15s

This image is courtesy of an anonymous Abreview

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (AB126734)
  • WB

Lab

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (AB126734)

Lanes 1-4 : Merged signal (red and green). Green - ab126734 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

ab126734 Anti-SNF5/SMARCB1 antibody [EPR6966] was shown to specifically react with SNF5/SMARCB1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267269 (knockout cell lysate ab257688) was used. Wild-type and SNF5/SMARCB1 knockout samples were subjected to SDS-PAGE. ab126734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

SMARCB1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human SMARCB1 (SNF5) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-smarcb1-snf5-knockout-hek-293t-cell-line-ab267269'>ab267269</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 44 kDa,84 kDa

Observed band size: 50 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6966

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNF5/SMARCB1 also known as INI1 or BAF47 is a member of the SWI/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5/SMARCB1 facilitates transcriptional regulation and chromosomal stability.
Biological function summary

SNF5/SMARCB1 acts as an integral component of the SWI/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.

Pathways

The involvement of SNF5/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.

SNF5/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5/SMARCB1 disrupts the SWI/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.
See full target information SMARCB1
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 150: PubMed37294080

2023

A chromatin remodelling SWI/SNF subunit, Snr1, regulates neural stem cell determination and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie E Keegan,Julie Haskins,Andrew J Simmonds,Sarah C Hughes

Med (New York, N.Y.) 3:774-791.e7 PubMed36195086

2022

Validation of a non-oncogene encoded vulnerability to exportin 1 inhibition in pediatric renal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Diego F Coutinho,Prabhjot S Mundi,Lianna J Marks,Chelsey Burke,Michael V Ortiz,Daniel Diolaiti,Lauren Bird,Kelly L Vallance,Glorymar Ibáñez,Daoqi You,Matthew Long,Nestor Rosales,Adina Grunn,Andoyo Ndengu,Armaan Siddiquee,Ervin S Gaviria,Allison R Rainey,Ladan Fazlollahi,Hajime Hosoi,Andrea Califano,Andrew L Kung,Filemon S Dela Cruz

Nature genetics 51:1399-1410 PubMed31427792

2019

Systematic characterization of BAF mutations provides insights into intracomplex synthetic lethalities in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Schick,André F Rendeiro,Kathrin Runggatscher,Anna Ringler,Bernd Boidol,Melanie Hinkel,Peter Májek,Loan Vulliard,Thomas Penz,Katja Parapatics,Christian Schmidl,Jörg Menche,Guido Boehmelt,Mark Petronczki,André C Müller,Christoph Bock,Stefan Kubicek

Scientific reports 8:15369 PubMed30337622

2018

smarce1 mutants have a defective endocardium and an increased expression of cardiac transcription factors in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Castillo-Robles,Laura Ramírez,Herman P Spaink,Hilda Lomelí

The Journal of biological chemistry 291:18058-18071 PubMed27382057

2016

Chromatin Remodeler Recruitment during Macrophage Differentiation Facilitates Transcription Factor Binding to Enhancers in Mature Cells.

Applications

ChIP

Species

Mouse

Michael J McAndrew,Alison Gjidoda,Mohita Tagore,Tyler Miksanek,Monique Floer

British journal of cancer 109:2751-62 PubMed24169358

2013

Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.

Applications

Unspecified application

Species

Human

B Giessrigl,W M Schmidt,M Kalipciyan,M Jeitler,M Bilban,M Gollinger,S Krieger,W Jäger,R M Mader,G Krupitza
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB88589

Anti-SNF5/SMARCB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SNF5/SMARCB1 antibody (AB88589)

  • 0
  • 0
Primary Antibodies

AB181976

Anti-SNF5/SMARCB1 antibody [EPR12014]

RabMAb|Recombinant

Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] (AB181976)

  • 0
  • 0
Primary Antibodies

AB192864

Anti-SNF5/SMARCB1 antibody [EPR12014-77]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (AB192864)

  • 0
  • 0
Primary Antibodies

AB222519

Anti-SNF5/SMARCB1 antibody [EPR20189]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-SNF5/SMARCB1 antibody [EPR20189] (AB222519)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

  • 5
  • 10
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com