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Rabbit Multiclonal SNF5/SMARCB1 antibody. Suitable for WB, ChIP and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCB1.

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Images

ChIP - Anti-SMARCB1 antibody [RP23040266] - ChIP Grade (AB307985), expandable thumbnail
  • Western blot - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (AB307985), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Recombinant Fragment Protein within Human SMARCB1. Database link Q12824

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBChIP
Human
Tested
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/250

Notes

-

Species

Mouse

Dilution info

1/250

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

4 μg on sheared chromatin from 2 million HEK cells.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

Alternative names

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, Integrase interactor 1 protein, SNF5 homolog, BAF47, hSNF5, SMARCB1, SNF5L1, BAF47, INI1

Recommended products

Rabbit Multiclonal SNF5/SMARCB1 antibody. Suitable for WB, ChIP and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SMARCB1.

Alternative names

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, BRG1-associated factor 47, Integrase interactor 1 protein, SNF5 homolog, BAF47, hSNF5, SMARCB1, SNF5L1, BAF47, INI1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Recombinant Fragment Protein within Human SMARCB1. Database link Q12824
Clone number

RP23040266

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SNF5/SMARCB1 also known as INI1 or BAF47 is a member of the SWI/SNF chromatin remodeling complex. It acts in modifying chromatin structure to regulate gene expression. SNF5/SMARCB1 possesses a mass of approximately 47 kDa. The protein expresses ubiquitously in various tissues with notable presence in the nucleus. Through its mechanical activity in chromatin remodeling SNF5/SMARCB1 facilitates transcriptional regulation and chromosomal stability.

Biological function summary

SNF5/SMARCB1 acts as an integral component of the SWI/SNF complex. This complex functions in altering nucleosome positioning on DNA impacting gene expression linked to cell cycle regulation differentiation and DNA repair. By working within this complex SNF5/SMARCB1 helps in the activation or repression of specific gene sets based on cellular requirements displaying an essential role in maintaining normal cellular function. The interplay of SNF5/SMARCB1 with other chromatin-modifying enzymes extends its influence across various genetic pathways.

Pathways

The involvement of SNF5/SMARCB1 in the cell cycle and transcription regulation pathways is acknowledged. It participates intimately in the p53 signaling pathway which is critical for cell cycle checkpoints and apoptotic responses. SNF5/SMARCB1 engages with other proteins such as p53 and RB1 coordinating to maintain genomic integrity and proper transcriptional programming. These pathways spotlight the protein's influence in important cell fate decisions.

Associated diseases and disorders

SNF5/SMARCB1 mutations or deletions associate closely with malignant rhabdoid tumors an aggressive pediatric cancer and schwannomatosis a disorder involving benign nerve sheath tumors. The loss of SNF5/SMARCB1 disrupts the SWI/SNF complex's function triggering unregulated cell proliferation and cancer progression. Within these disorders the SWI/SNF complex itself which contains other subunits like BRG1 and BRM is also impacted highlighting SMARCB1's critical role in tumor suppression.

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2 product images

  • ChIP - Anti-SMARCB1 antibody [RP23040266] - ChIP Grade (ab307985), expandable thumbnail

    ChIP - Anti-SMARCB1 antibody [RP23040266] - ChIP Grade (ab307985)

    Enrichment of endogenous SMARCB1 protein at specific gene loci using Anti-SMARCB1 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-SMARCB1 antibody (ab307985) at 4 μg on sheared chromatin from 2 million HEK cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoters of CDKN1A, ESR1 (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

  • Western blot - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (ab307985), expandable thumbnail

    Western blot - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (ab307985)

    Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), MOLT-4 (Lane 4), A549 (Lane 5), U-2 OS (Lane 6), LNCaP (Lane 7), NIH/3T3 (Lane 8) and tissue extract of Mouse Testis (Lane 9). The blot was probed with ab307985 at 1:250 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1:4000 dilution. A ~44 kDa band corresponding to SMARCB1 was observed across the cell lines and tissue tested.

    All lanes: Western blot - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (ab307985) at 1/250 dilution

    Lane 1: HEK-293 whole cell lysate at 30 µg

    Lane 2: HeLa whole cell lysate at 30 µg

    Lane 3: Jurkat whole cell lysate at 30 µg

    Lane 4: MOLT-4 whole cell lysate at 30 µg

    Lane 5: A549 whole cell lysate at 30 µg

    Lane 6: U-2 OS whole cell lysate at 30 µg

    Lane 7: LNCaP whole cell lysate at 30 µg

    Lane 8: NIH/3T3 whole cell lysate at 30 µg

    Lane 9: Mouse testis tissue lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

    Developed using the ECL technique.

    Observed band size: 44 kDa

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Product protocols

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