Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade

• ChIP

Supplier Data

ChIP - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (AB307985)

Enrichment of endogenous SMARCB1 protein at specific gene loci using Anti-SMARCB1 Antibody : Chromatin Immunoprecipitation (ChIP) was performed using Anti-SMARCB1 antibody (ab307985) at 4 μg on sheared chromatin from 2 million HEK cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoters of CDKN1A, ESR1 (positive) and SAT2 satellite repeats (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

• WB

Supplier Data

Western blot - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (AB307985)

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), MOLT-4 (Lane 4), A549 (Lane 5), U-2 OS (Lane 6), LNCaP (Lane 7), NIH/3T3 (Lane 8) and tissue extract of Mouse Testis (Lane 9). The blot was probed with ab307985 at 1 : 250 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1 : 4000 dilution. A ~44 kDa band corresponding to SMARCB1 was observed across the cell lines and tissue tested.

All lanes:

Western blot - Anti-SNF5/SMARCB1 antibody [RP23040266] - ChIP Grade (ab307985) at 1/250 dilution

Lane 1:

HEK-293 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

Jurkat whole cell lysate at 30 µg

Lane 4:

MOLT-4 whole cell lysate at 30 µg

Lane 5:

A549 whole cell lysate at 30 µg

Lane 6:

U-2 OS whole cell lysate at 30 µg

Lane 7:

LNCaP whole cell lysate at 30 µg

Lane 8:

NIH/3T3 whole cell lysate at 30 µg

Lane 9:

Mouse testis tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/4000 dilution

Observed band size: 44 kDa

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