AB126194

Anti-SNIP1 antibody

Name: Anti-SNIP1 antibody (ab126194) | Abcam
Brand: Abcam Limited
SKU: AB126194
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal SNIP1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNIP1 aa 1-350.

View Alternative Names

Smad nuclear-interacting protein 1, FHA domain-containing protein SNIP1, SNIP1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

ab126194, at 1/500 dilution, staining SNIP1 in Paraffin-embedded HumRat RT2 xenograft tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

ab126194, at 1/500 dilution, staining SNIP1 in Paraffin-embedded Human 59T xenograft tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

ab126194, at 1/500 dilution, staining SNIP1 in Paraffin-embedded Mouse Muscle tissue by Immunohistochemistry.

Unknown

Western blot - Anti-SNIP1 antibody (AB126194)

10% SDS PAGE

All lanes:

Western blot - Anti-SNIP1 antibody (ab126194) at 1/5000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 40 kDa,46 kDa,47 kDa,48 kDa,49 kDa,54 kDa,59 kDa,68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SNIP1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q8TAD8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNIP1 also known as Smad nuclear interacting protein 1 is a nuclear protein with a mass of approximately 43 kDa. This protein mechanically functions as a nuclear co-repressor that interacts with Smad proteins and the transcription factor c-Myc. SNIP1 is expressed in various tissues notably in embryonic and adult brain tissues which indicates a role in development and cellular regulation within these areas.

Biological function summary

SNIP1 influences transcriptional repression by modulating the transcriptional activity of the Smad4 complex. SNIP1 interacts with several regulatory proteins to modulate gene expression. By inhibiting the transcriptional activity of Smad proteins SNIP1 can regulate various genes involved in cell growth and proliferation affecting how cells behave in different contexts.

Pathways

SNIP1 participates in important cellular signaling pathways such as the TGF-beta and BMP signaling pathways. SNIP1 interacts with proteins in these pathways including Smad2 and Smad3 to control cellular responses to growth factors. By influencing these pathways SNIP1 plays a part in cellular processes like apoptosis and differentiation.

SNIP1 has implications in cancer and neurodevelopmental disorders. In cancer SNIP1's interaction with proteins like c-Myc and Smad3 plays a role in tumor progression highlighting its involvement in oncogenic pathways. In neurodevelopmental disorders altered SNIP1 expression affects normal brain development suggesting links between its expression and conditions such as autism spectrum disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 29360106). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.

See full target information SNIP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2192 PubMed35449131

2022

KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yu,Jun Su,Qiqi Shi,Qing Liu,Jun Ma,Guoqing Ru,Lei Zhang,Jian Zhang,Xichun Hu,Jianming Tang

American journal of human genetics 109:116-135 PubMed34965383

2021

Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Nakamura,Brett M Reid,Ann Chen,Zhihua Chen,Ellen L Goode,Jennifer B Permuth,Jamie K Teer,Jonathan Tyrer,Xiaoqing Yu,Peter A Kanetsky,Paul D Pharoah,Simon A Gayther,Thomas A Sellers,Kate Lawrenson,Florian A Karreth

Cell death & disease 12:576 PubMed34088891

2021

Cancer-secreted exosomal miR-21-5p induces angiogenesis and vascular permeability by targeting KRIT1.

Applications

Unspecified application

Species

Unspecified reactive species

Qinglian He,Aihua Ye,Weibiao Ye,Xiaomin Liao,Guoqiang Qin,Yongqiang Xu,Yuting Yin,Huanqian Luo,Muhua Yi,Liying Xian,Shihao Zhang,Xiyuan Qin,Wei Zhu,Yuling Li

Scandinavian journal of immunology 93:e13007 PubMed33264420

2020

Upregulated microRNA-132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Feng,Zilin Cui,Zirong Liu,Yamin Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SNIP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNIP1 antibody (AB126194)

Western blot - Anti-SNIP1 antibody (AB126194)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise