AB251140

Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SNPH antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

KIAA0374, SNPH, Syntaphilin

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

This data was developed using ab192605, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

This data was developed using ab192605, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

This data was developed using ab192605, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNPH antibody [EPR14115(2)] (<a href='/en-us/products/primary-antibodies/snph-antibody-epr141152-ab192605'>ab192605</a>) at 1/10000 dilution

All lanes:

Human hippocamus tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

This data was developed using ab192605, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNPH antibody [EPR14115(2)] (<a href='/en-us/products/primary-antibodies/snph-antibody-epr141152-ab192605'>ab192605</a>) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

This data was developed using ab192605, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNPH antibody [EPR14115(2)] (<a href='/en-us/products/primary-antibodies/snph-antibody-epr141152-ab192605'>ab192605</a>) at 1/5000 dilution

All lanes:

Rat brain at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

false

Unconjugated

Anti-SNPH antibody [EPR14115(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14115(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251140 is the carrier-free version of ab192605.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNPH also known as Syntaphilin is a protein that acts mechanically by binding to microtubules which is important for its role in anchoring mitochondria within axons. It has a molecular mass of approximately 66 kDa. SNPH predominantly expresses in neurons particularly in the brain suggesting its significant role in the nervous system. This anchoring function helps to regulate energy distribution and calcium buffering within axons highlighting its importance in maintaining proper neuronal function.

Biological function summary

This protein modulates mitochondrial distribution by stabilizing microtubules and interacting with the mitochondrial outer membrane. SNPH operates as a part of a complex with motor proteins and microtubular structures which facilitates the stationary positioning of mitochondria along the axons. It helps in energy homeostasis and signal transmission supporting synaptic activity and neuronal health. By influencing mitochondrial dynamics SNPH ensures that neurons maintain energy efficiency and health.

Pathways

SNPH plays a role in cellular processes like the mitochondrial transport and microtubular dynamics pathway. This involves SNPH interacting with proteins such as kinesin and dynein which are motor proteins that move cellular cargo along microtubules. SNPH helps anchor mitochondria by counteracting these transport proteins which helps in the proper positioning of mitochondria needed for neuron function. SNPH's activity is connected to the intracellular energy distribution pathway influencing the energy supply necessary for high-demand areas within the neurons.

SNPH is associated with neurodegenerative conditions such as Alzheimer’s disease and Parkinson’s disease. These diseases feature mitochondrial dysfunction and energy imbalances where SNPH’s role in mitochondrial anchoring and distribution becomes disrupted contributing to disease advancement. SNPH’s interactions with amyloid precursor protein in Alzheimer's and alpha-synuclein in Parkinson’s have shown relevance in the pathological processes of these disorders. Understanding SNPH’s function offers insights into therapeutic approaches targeting mitochondrial dynamics in neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits SNARE complex formation by absorbing free STX1A.

See full target information SNPH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

Western blot - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

Western blot - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

Western blot - Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (AB251140)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise