AB176715

Anti-SNRNP200 antibody

Name: Anti-SNRNP200 antibody (ab176715) | Abcam
Brand: Abcam Limited
SKU: AB176715

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(4 Publications)

Rabbit Polyclonal SNRNP200 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SNRNP200 aa 400-450.

View Alternative Names

ASCC3L1, BRR2, HELIC2, KIAA0788, SNRNP200, U5 small nuclear ribonucleoprotein 200 kDa helicase, Activating signal cointegrator 1 complex subunit 3-like 1, BRR2 homolog, U5 snRNP-specific 200 kDa protein, U5-200KD

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP200 antibody (AB176715)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP200 antibody (AB176715)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections using ab176715 at 1/1000 dilution (1 μg/mL).

Supplier Data

Immunoprecipitation - Anti-SNRNP200 antibody (AB176715)

SNRNP200 was immunoprecipitated from 1mg HeLa whole cell lysate using ab176715 at 6 μg/mg lysate (Lane 2), a rabbit anti-SNRNP200 antibody recognizing an upstream epitope (Lane 1) or control IgG (Lane 3). 20% of the Immunoprecipitate was loaded per lane and then immunobotted using ab176715 at 1.0 μg/ml.

Detection :

Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SNRNP200 antibody (ab176715)

Predicted band size: 245 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-SNRNP200 antibody (AB176715)

All lanes:

Western blot - Anti-SNRNP200 antibody (ab176715) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 245 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human SNRNP200 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

O75643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176715 was affinity purified using an epitope specific to SNRNP200 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRNP200 also known as BRR2 or hBrr2 is an essential part of the spliceosome machinery. It is a large protein with a mass of approximately 250 kDa. The protein is widely expressed in various tissues with high levels in cells that have robust splicing activity. SNRNP200 is an ATPase and helicase and its mechanical role involves unwinding RNA duplexes during pre-mRNA splicing. Its activity is necessary for the rearrangements within the spliceosome facilitating the transition between different stages of the splicing cycle.

Biological function summary

SNRNP200 facilitates pre-mRNA splicing by being part of the U5 small nuclear ribonucleoprotein (snRNP) complex. The protein helps in the unwinding of RNA helices allowing snRNPs to correctly assemble and disassemble on pre-mRNA substrates. Efficient pre-mRNA splicing by SNRNP200 ensures accurate removal of introns and joining of exons leading to mature mRNA production. SNRNP200 collaborates with other spliceosomal components to maintain fidelity in the splicing process which is important for gene expression regulation.

Pathways

SNRNP200 plays integral roles in the splicing pathway and RNA processing pathway. Within the splicing pathway it interacts closely with several other spliceosomal proteins including PRPF8 and EFTUD2 to modulate spliceosome dynamics. These interactions facilitate the precise and timely execution of splicing events. The RNA processing pathway involves intricate networks of proteins where SNRNP200's helicase activity is critical for processing precursor mRNAs into mature forms for translation.

Abnormalities associated with SNRNP200 have been linked to retinitis pigmentosa and certain forms of glaucoma. Mutations in the gene encoding SNRNP200 can lead to defects in RNA splicing which in turn disrupts the expression of proteins necessary for retinal health and function. This protein also interacts with other proteins like PRPF31 and PRPF8 which are associated with similar retinal degenerative disorders. Understanding SNRNP200's role in these diseases provides insight into the molecular mechanisms underlying specific vision-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome (PubMed : 35241646). Plays a role in pre-mRNA splicing as a core component of precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed : 28502770, PubMed : 28781166, PubMed : 29301961, PubMed : 29360106, PubMed : 29361316, PubMed : 30315277, PubMed : 30705154, PubMed : 30728453). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome.

See full target information SNRNP200

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Skeletal muscle 14:30 PubMed39627769

2024

SIX transcription factors are necessary for the activation of DUX4 expression in facioscapulohumeral muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Amelia Fox,Jonathan Oliva,Rajanikanth Vangipurapu,Francis M Sverdrup

Cell discovery 10:96 PubMed39285160

2024

Targeting SNRNP200-induced splicing dysregulation offers an immunotherapy opportunity for glycolytic triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiao Yang,Luo Hong,Linwei Guo,Yunjin Wang,Xiangchen Han,Boyue Han,Zheng Xing,Guoliang Zhang,Hongxia Zhou,Chao Chen,Hong Ling,Zhimin Shao,Xin Hu

Human molecular genetics 32:319-332 PubMed35994036

2022

Stress granule assembly in vivo is deficient in the CNS of mutant TDP-43 ALS mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Dubinski,Myriam Gagné,Sarah Peyrard,David Gordon,Kevin Talbot,Christine Vande Velde

BMC developmental biology 20:22 PubMed33203369

2020

Depletion of SNRNP200 inhibits the osteo-/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Su,Haoqing Yang,Ruitang Shi,Chen Zhang,Huina Liu,Zhipeng Fan,Jianpeng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SNRNP200 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP200 antibody (AB176715)

Immunoprecipitation - Anti-SNRNP200 antibody (AB176715)

Western blot - Anti-SNRNP200 antibody (AB176715)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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