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AB254796

Anti-SNRNP40 antibody

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(1 Publication)

Rabbit Polyclonal SNRNP40 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SNRNP40 aa 250-400.

View Alternative Names

PRP8BP, SFP38, WDR57, SNRNP40, U5 small nuclear ribonucleoprotein 40 kDa protein, U5 snRNP 40 kDa protein, U5-40K, 38 kDa-splicing factor, Prp8-binding protein, U5 snRNP-specific 40 kDa protein, WD repeat-containing protein 57, hPRP8BP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP40 antibody (AB254796)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP40 antibody (AB254796)

Paraffin-embedded human duodenum tissue stained for SNRNP40 using ab254796 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SNRNP40 antibody (AB254796)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SNRNP40 antibody (AB254796)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling SNRNP40 using ab254796 at 4µg/ml (green) in ICC/IF.

Western blot - Anti-SNRNP40 antibody (AB254796)
  • WB

Supplier Data

Western blot - Anti-SNRNP40 antibody (AB254796)

All lanes:

Western blot - Anti-SNRNP40 antibody (ab254796) at 0.4 µg/mL

Lane 1:

NIH/3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SNRNP40 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q96DI7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRNP40 also known as U5-40K or Splicing Factor 40 kDa is a component of the U5 small nuclear ribonucleoprotein complex. It has a mass of approximately 40 kDa. SNRNP40 is mainly expressed in the nucleoplasm of eukaryotic cells where it plays a critical role in RNA processing.
Biological function summary

SNRNP40 participates in the pre-mRNA splicing process as part of the spliceosome a large and dynamic ribonucleoprotein complex. The spliceosome facilitates the removal of introns from pre-mRNA transcripts. SNRNP40 as a part of this complex influences the conformational changes necessary for the accurate assembly and function of the spliceosome. Through this action SNRNP40 helps maintain genomic stability and proper gene expression.

Pathways

SNRNP40's role in the spliceosome places it within essential RNA processing pathways including the mRNA splicing pathway. It interacts with other proteins such as PRPF8 and EFTUD2 (also known as Snu114) which are key components of the U5 particle. These interactions illustrate how SNRNP40's activity is integral to the functioning of the spliceosomal machinery.

SNRNP40 has been connected to certain genetic conditions through its role in RNA splicing. Defects in the splicing process can result in splicing-related disorders such as retinitis pigmentosa although SNRNP40 itself is not directly implicated in this disease. However it shares functional associations with proteins like PRPF31 which is linked to such disorders. Additionally SNRNP40 may play a role in cancer development due to its involvement in mis-splicing events affecting tumor suppressor genes and oncogenes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for pre-mRNA splicing as component of the activated spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 29301961, PubMed : 29360106, PubMed : 30315277, PubMed : 30705154). Component of the U5 small nuclear ribonucleoprotein (snRNP) complex and the U4/U6-U5 tri-snRNP complex, building blocks of the spliceosome (PubMed : 16723661, PubMed : 26912367, PubMed : 9774689). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
See full target information SNRNP40
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 19:246 PubMed34404391

2021

Identification of a novel circ_0018289/miR-183-5p/TMED5 regulatory network in cervical cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zou,Huijia Chen,Shuaibin Liu,Xiaoling Gan
View all publications
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Product promise

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