Anti-SNRP70/U1-70K antibody (ab83306) is a rabbit polyclonal antibody that is used to detect SNRP70/U1-70K in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 20 publications
- Trusted since 2010
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- Nature 618:180-1872023Mitotic bookmarking by SWI/SNF subunits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhexin Zhu,Xiaolong Chen,Ao Guo,Trishabelle Manzano,Patrick J Walsh,Kendall M Wills,Rebecca Halliburton,Sandi Radko-Juettner,Raymond D Carter,Janet F Partridge,Douglas R Green,Jinghui Zhang,Charles W M RobertsPubMed 37225980
- Nature communications 14:9572023LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang Zhu,Kang Chen,Yu H Sun,Wen Ye,Juntao Liu,Dandan Zhang,Nan Su,Li Wu,Xiaochen Kou,Yanhong Zhao,Hong Wang,Shaorong Gao,Lan KangPubMed 36810573
- Cell reports 39:1109442022Analysis of estrogen-regulated enhancer RNAs identifies a functional motif required for enhancer assembly and gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTim Y Hou,W Lee KrausPubMed 35705040
- Disease models & mechanisms 15:2022Physiological tissue-specific and age-related reduction of mouse TDP-43 levels is regulated by epigenetic modifications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam Pacetti,Laura De Conti,Luciano E Marasco,Maurizio Romano,Mohammad M Rashid,Martina Nubiè,Francisco E Baralle,Marco BarallePubMed 35243489
- RNA (New York, N.Y.) 28:878-8942022ZFC3H1 and U1-70K promote the nuclear retention of mRNAs with 5' splice site motifs within nuclear speckles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEliza S Lee,Harrison W Smith,Eric J Wolf,Aysegul Guvenek,Yifan E Wang,Andrew Emili,Bin Tian,Alexander F PalazzoPubMed 35351812
- Frontiers in oncology 11:6383972022SETMAR Shorter Isoform: A New Prognostic Factor in Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOriane Lié,Thierry Virolle,Mathieu Gabut,Claude Pasquier,Ilyess Zemmoura,Corinne Augé-GouillouPubMed 35047379
- The Journal of biological chemistry 298:1015902022The METTL5-TRMT112 N-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaraline Sepich-Poore,Zhong Zheng,Emily Schmitt,Kailong Wen,Zijie Scott Zhang,Xiao-Long Cui,Qing Dai,Allen C Zhu,Linda Zhang,Arantxa Sanchez Castillo,Haiyan Tan,Junmin Peng,Xiaoxi Zhuang,Chuan He,Sigrid NachtergaelePubMed 35033535
- Molecular cell :2021Stress-induced nuclear condensation of NELF drives transcriptional downregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrashant Rawat et. al.PubMed 33548202
- eLife 10:2021Identification of PARP-7 substrates reveals a role for MARylation in microtubule control in ovarian cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLavanya H Palavalli Parsons et. al.PubMed 33475085
- International journal of oncology 56:508-5212020Long non‑coding RNA LINC00473/miR‑195‑5p promotes glioma progression via YAP1‑TEAD1‑Hippo signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Wang,Xu Dong Li,Zhenyuan Fu,Yan Zhou,Xing Huang,Xiaobing JiangPubMed 31894297
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Target data
Function
Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome (PubMed:19325628, PubMed:25555158). SNRNP70 binds to the loop I region of U1-snRNA (PubMed:19325628, PubMed:2467746, PubMed:25555158). Isoform 3. Truncated isoforms that lack the RRM domain cannot bind U1-snRNA. Isoform 4. Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.
Alternative names
RNPU1Z, RPU1, SNRP70, U1AP1, SNRNP70, U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, U1-70K, snRNP70
Recommended products
Anti-SNRP70/U1-70K antibody (ab83306) is a rabbit polyclonal antibody that is used to detect SNRP70/U1-70K in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 20 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of ab83306 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-SNRP70/U1-70K antibody (ab83306) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.
What is the molecular weight of SNRP70/U1-70K?
Anti-SNRP70/U1-70K (ab83306) specifically detects a band for SNRP70/U1-70K (UniProt: P08621) at a molecular weight of 51kDa.
Recommended positive controls
WB: HeLa; Jurkat; HepG2; HEK293; CaCo2; MCF7; SHSY5Y; NIH3T3; MEF1 whole cell lysates. Mouse and Rat testis tissue lysates. IHC-P: Normal Human Colon TissueICC/IF: HeLa
Trusted by the scientific community
Anti-SNRP70/U1-70K (ab83306) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNRP70 also known as U1-70K is a protein component of the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) complex. It has a molecular mass of approximately 70 kDa. This protein is mainly expressed in the nucleus of eukaryotic cells. SNRP70 binds to the U1 snRNA facilitating the early stages of pre-mRNA splicing. It stabilizes the binding of the U1 snRNP to the 5' splice site therefore playing a role in recognizing and defining correct splice sites necessary for mRNA maturation.
- Biological function summary
This protein is integral to the spliceosome a large multi-component complex responsible for removing introns from pre-mRNA. SNRP70 interacts with other core components of the U1 snRNP such as U1A and U1C proteins to assemble the complex. It ensures the accuracy of splice site recognition and contributes to the dynamic rearrangement of the spliceosome during splicing. These interactions importantly influence the regulation of gene expression by facilitating alternative splicing which allows a single gene to code for multiple proteins.
- Pathways
SNRP70 is actively involved in the mRNA processing pathway a critical process in gene expression regulation. Its function directly links to the RNA splicing pathway ensuring the generation of mature mRNA from precursor transcripts. SNRP70's role in the spliceosomal complex places it in association with other spliceosome-related proteins such as SF3B1. This interconnectedness with SF3B1 supports the protein's engagement in ensuring accurate splicing and modulating gene expression through alternative splicing events.
- Associated diseases and disorders
Disruptions in SNRP70 function associate with certain autoimmune conditions and neurodegenerative diseases. For instance autoantibodies against SNRP70 are characteristic in patients with systemic lupus erythematosus (SLE) an autoimmune disorder. Also aberrant splicing events involving SNRP70 contribute to the pathology of certain neurodegenerative diseases such as spinal muscular atrophy. In these conditions the misregulation of splicing factors can exacerbate disease progression linking SNRP70 with other autoantigen proteins like Sm proteins in SLE.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-SNRP70/U1-70K antibody (ab83306)
The 63 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human U1 small nuclear ribonucleoprotein 70 kDa.
All lanes: Western blot - Anti-SNRP70/U1-70K antibody (ab83306) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 5: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 63 kDa
Exposure time: 2min
Western blot - Anti-SNRP70/U1-70K antibody (ab83306)
All lanes: Western blot - Anti-SNRP70/U1-70K antibody (ab83306) at 1 µg/mL
Lane 1: Testis (Rat) Tissue Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Testis (Mouse) Tissue Lysate at 10 µg
Lane 4: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 24 kDa, 63 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody (ab83306)
IHC image of SNRP70/U1-70K antibody staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab83306, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry/ Immunofluorescence - Anti-SNRP70/U1-70K antibody (ab83306)
ab83306 staining SNRP70/U1-70K in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab83306 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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