AB316763

Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal SNRP70/U1-70K antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

RNPU1Z, RPU1, SNRP70, U1AP1, SNRNP70, U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, U1-70K, snRNP70

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SNRP70/U1-70K with ab316762 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon.
The section was incubated with ab316762 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling SNRP70/U1-70K with ab316762 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat lung.
The section was incubated with ab316762 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SNRP70/U1-70K with ab316762 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse colon.
The section was incubated with ab316762 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SNRP70/U1-70K with ab316762 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat colon.
The section was incubated with ab316762 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] (<a href='/en-us/products/primary-antibodies/snrp70-u1-70k-antibody-epr28616-89-ab316762'>ab316762</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

Hela transfected with siRNA specifically targeti SNRP70/U1-70K whole cell lysate at 40 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Lane 4:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 5:

MCF7(human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] (<a href='/en-us/products/primary-antibodies/snrp70-u1-70k-antibody-epr28616-89-ab316762'>ab316762</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 40 µg

Lane 2:

Rat breast tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 70 kDa

true

Exposure time: 92s

Supplier Data

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

This data was developed using ab316762, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] (<a href='/en-us/products/primary-antibodies/snrp70-u1-70k-antibody-epr28616-89-ab316762'>ab316762</a>) at 1/1000 dilution

Lane 1:

Rat lung tissue lysate at 80 µg

Lane 2:

Mouse lung tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 70 kDa

true

Exposure time: 26s

Unconjugated

Anti-SNRP70/U1-70K antibody [EPR28616-89]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28616-89

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab316763 is the carrirer-free version of ab316762.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRP70 also known as U1-70K is a protein component of the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) complex. It has a molecular mass of approximately 70 kDa. This protein is mainly expressed in the nucleus of eukaryotic cells. SNRP70 binds to the U1 snRNA facilitating the early stages of pre-mRNA splicing. It stabilizes the binding of the U1 snRNP to the 5' splice site therefore playing a role in recognizing and defining correct splice sites necessary for mRNA maturation.

Biological function summary

This protein is integral to the spliceosome a large multi-component complex responsible for removing introns from pre-mRNA. SNRP70 interacts with other core components of the U1 snRNP such as U1A and U1C proteins to assemble the complex. It ensures the accuracy of splice site recognition and contributes to the dynamic rearrangement of the spliceosome during splicing. These interactions importantly influence the regulation of gene expression by facilitating alternative splicing which allows a single gene to code for multiple proteins.

Pathways

SNRP70 is actively involved in the mRNA processing pathway a critical process in gene expression regulation. Its function directly links to the RNA splicing pathway ensuring the generation of mature mRNA from precursor transcripts. SNRP70's role in the spliceosomal complex places it in association with other spliceosome-related proteins such as SF3B1. This interconnectedness with SF3B1 supports the protein's engagement in ensuring accurate splicing and modulating gene expression through alternative splicing events.

Disruptions in SNRP70 function associate with certain autoimmune conditions and neurodegenerative diseases. For instance autoantibodies against SNRP70 are characteristic in patients with systemic lupus erythematosus (SLE) an autoimmune disorder. Also aberrant splicing events involving SNRP70 contribute to the pathology of certain neurodegenerative diseases such as spinal muscular atrophy. In these conditions the misregulation of splicing factors can exacerbate disease progression linking SNRP70 with other autoantigen proteins like Sm proteins in SLE.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome (PubMed : 19325628, PubMed : 25555158). SNRNP70 binds to the loop I region of U1-snRNA (PubMed : 19325628, PubMed : 2467746, PubMed : 25555158).. Isoform 3. Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.. Isoform 4. Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.

See full target information SNRNP70

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Western blot - Anti-SNRP70/U1-70K antibody [EPR28616-89] - BSA and Azide free (AB316763)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise