JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB128937

Anti-SNRPA1 antibody [EPR7557]

1

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal SNRPA1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

U2 small nuclear ribonucleoprotein A', U2 snRNP A', SNRPA1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] (AB128937)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] (AB128937)

ab128937 at a 1/100 dilution staining SNRPA1 in paraffin-embedded Human squamous lung cell carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] (AB128937)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] (AB128937)

ab128937 at a 1/100 dilution staining SNRPA1 in paraffin-embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-SNRPA1 antibody [EPR7557] (AB128937)
  • WB

Unknown

Western blot - Anti-SNRPA1 antibody [EPR7557] (AB128937)

All lanes:

Western blot - Anti-SNRPA1 antibody [EPR7557] (ab128937) at 1/1000 dilution

Lane 1:

Huvec cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Rat liver tissue lysate at 10 µg

Lane 5:

Mouse liver tissue lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

OI-RD Scanning - Anti-SNRPA1 antibody [EPR7557] (AB128937)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SNRPA1 antibody [EPR7557] (AB128937)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7557

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPA1 also known as U1A is an essential protein component of the spliceosomal small nuclear ribonucleoprotein particle U1 snRNP. It has a molecular mass of approximately 31 kDa. This protein mainly facilitates the initial stages of mRNA splicing by binding to the 5' splice site in pre-mRNA. SNRPA1 is highly expressed in the nucleus of eukaryotic cells where it contributes to the precise removal of introns from pre-mRNA.
Biological function summary

The small nuclear ribonucleoprotein-associated protein A1 plays an important role in the splicing of pre-mRNA. It is part of the U1 snRNP complex which is important for the recognition and selection of 5' splice sites in pre-mRNA transcripts. This protein aids in the assembly of the spliceosome the large ribonucleoprotein complex responsible for catalyzing the excision of introns. SNRPA1 ensures the fidelity and regulation of mRNA maturation affecting gene expression levels.

Pathways

The involvement of SNRPA1 in the spliceosome cycle places it within the post-transcriptional modification and gene expression regulation pathways. It is intricately related to other spliceosomal components like U2 snRNP which is essential for the branch point recognition in the splicing process. Together with proteins such as SF3B1 SNRPA1 plays a role in the coordination and timing of splice site recognition and catalysis during the splicing pathway.

SNRPA1's role in mRNA splicing links it to conditions like spinal muscular atrophy (SMA) and some cancers. Spinal muscular atrophy involving survival motor neuron (SMN) protein dysfunction can be influenced by perturbed alternative splicing processes where SNRPA1 contributes. In certain cancers aberrant splicing patterns involving SNRPA1 and other kinases may lead to oncogenic transformations indicating its importance in maintaining splicing accuracy during cell growth and division.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 27035939, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA (PubMed : 27035939, PubMed : 32494006, PubMed : 9716128).
See full target information SNRPA1
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:1496-1511.e7 PubMed38537639

2024

The splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey Damianov,Chia-Ho Lin,Jeffrey Huang,Lin Zhou,Yasaman Jami-Alahmadi,Parham Peyda,James Wohlschlegel,Douglas L Black

PloS one 17:e0265742 PubMed35385551

2022

Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity.

Applications

Unspecified application

Species

Unspecified reactive species

Mina Obuća,Zuzana Cvačková,Jan Kubovčiak,Michal Kolář,David Staněk

Nature communications 12:3646 PubMed34131137

2021

TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.

Applications

Unspecified application

Species

Unspecified reactive species

Klára Klimešová,Jitka Vojáčková,Nenad Radivojević,Franck Vandermoere,Edouard Bertrand,Celine Verheggen,David Staněk

Aging 13:2895-2911 PubMed33460399

2021

Identification of RNA-binding protein SNRPA1 for prognosis in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Penghui Yuan,Le Ling,Xintao Gao,Taotao Sun,Jianping Miao,Xianglin Yuan,Jihong Liu,Zhihua Wang,Bo Liu

BMC biology 17:39 PubMed31088452

2019

Biological and RNA regulatory function of MOV10 in mammalian germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqiang Fu,Suwen Tian,Huanhuan Tan,Caifeng Wang,Hanben Wang,Min Wang,Yuanyuan Wang,Zhen Chen,Yanfeng Wang,Qiuling Yue,Qiushi Xu,Shuya Zhang,Haixin Li,Jie Xie,Mingyan Lin,Mengcheng Luo,Feng Chen,Lan Ye,Ke Zheng

PLoS genetics 13:e1006633 PubMed28234895

2017

A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B.

Applications

ChIP

Species

Mouse

Tatiana A Soboleva,Brian J Parker,Maxim Nekrasov,Gene Hart-Smith,Ying Jin Tay,Wei-Quan Tng,Marc Wilkins,Daniel Ryan,David J Tremethick
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB131281

Anti-Exportin-5 antibody [EPR8453]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Exportin-5 antibody [EPR8453] (AB131281)

  • 4
  • 1
Primary Antibodies

AB11826

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (AB11826)

  • 4
  • 16
Primary Antibodies

AB133607

Anti-hnRNP C1/C2 antibody [EPNCIR152]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP C1/C2 antibody [EPNCIR152] (AB133607)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com