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Rabbit Recombinant Monoclonal SNRPA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (AB248231), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (AB248231), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (AB248231), expandable thumbnail
  • OI-RD Scanning - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (AB248231), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Rat
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:27035939, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA (PubMed:27035939, PubMed:32494006, PubMed:9716128).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SNRPA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7557
Purification technique
Affinity purification Protein A
Dissociation constant
7.6 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248231 is the carrier-free version of Anti-SNRPA1 antibody [EPR7557] ab128937.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SNRPA1 also known as U1A is an essential protein component of the spliceosomal small nuclear ribonucleoprotein particle U1 snRNP. It has a molecular mass of approximately 31 kDa. This protein mainly facilitates the initial stages of mRNA splicing by binding to the 5' splice site in pre-mRNA. SNRPA1 is highly expressed in the nucleus of eukaryotic cells where it contributes to the precise removal of introns from pre-mRNA.

Biological function summary

The small nuclear ribonucleoprotein-associated protein A1 plays an important role in the splicing of pre-mRNA. It is part of the U1 snRNP complex which is important for the recognition and selection of 5' splice sites in pre-mRNA transcripts. This protein aids in the assembly of the spliceosome the large ribonucleoprotein complex responsible for catalyzing the excision of introns. SNRPA1 ensures the fidelity and regulation of mRNA maturation affecting gene expression levels.

Pathways

The involvement of SNRPA1 in the spliceosome cycle places it within the post-transcriptional modification and gene expression regulation pathways. It is intricately related to other spliceosomal components like U2 snRNP which is essential for the branch point recognition in the splicing process. Together with proteins such as SF3B1 SNRPA1 plays a role in the coordination and timing of splice site recognition and catalysis during the splicing pathway.

Associated diseases and disorders

SNRPA1's role in mRNA splicing links it to conditions like spinal muscular atrophy (SMA) and some cancers. Spinal muscular atrophy involving survival motor neuron (SMN) protein dysfunction can be influenced by perturbed alternative splicing processes where SNRPA1 contributes. In certain cancers aberrant splicing patterns involving SNRPA1 and other kinases may lead to oncogenic transformations indicating its importance in maintaining splicing accuracy during cell growth and division.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231), expandable thumbnail

    Western blot - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231)

    This data was developed using Anti-SNRPA1 antibody [EPR7557] ab128937, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SNRPA1 antibody [EPR7557] (Anti-SNRPA1 antibody [EPR7557] ab128937) at 1/1000 dilution

    Lane 1: Huvec cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: Rat liver tissue lysate at 10 µg

    Lane 5: Mouse liver tissue lysate at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 28 kDa

    Observed band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231)

    This data was developed using Anti-SNRPA1 antibody [EPR7557] ab128937, the same antibody clone in a different buffer formulation.Anti-SNRPA1 antibody [EPR7557] ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231)

    This data was developed using Anti-SNRPA1 antibody [EPR7557] ab128937, the same antibody clone in a different buffer formulation.Anti-SNRPA1 antibody [EPR7557] ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human squamous lung cell carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231), expandable thumbnail

    OI-RD Scanning - Anti-SNRPA1 antibody [EPR7557] - BSA and Azide free (ab248231)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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