Rabbit Recombinant Monoclonal SNRPA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: PBS
IHC-P | Flow Cyt | WB | |
---|---|---|---|
Human | Tested | Not recommended | Tested |
Mouse | Expected | Not recommended | Tested |
Rat | Expected | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:27035939, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA (PubMed:27035939, PubMed:32494006, PubMed:9716128).
U2 small nuclear ribonucleoprotein A', U2 snRNP A', SNRPA1
Rabbit Recombinant Monoclonal SNRPA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: PBS
ab248231 is the carrier-free version of Anti-SNRPA1 antibody [EPR7557] ab128937.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
SNRPA1 also known as U1A is an essential protein component of the spliceosomal small nuclear ribonucleoprotein particle U1 snRNP. It has a molecular mass of approximately 31 kDa. This protein mainly facilitates the initial stages of mRNA splicing by binding to the 5' splice site in pre-mRNA. SNRPA1 is highly expressed in the nucleus of eukaryotic cells where it contributes to the precise removal of introns from pre-mRNA.
The small nuclear ribonucleoprotein-associated protein A1 plays an important role in the splicing of pre-mRNA. It is part of the U1 snRNP complex which is important for the recognition and selection of 5' splice sites in pre-mRNA transcripts. This protein aids in the assembly of the spliceosome the large ribonucleoprotein complex responsible for catalyzing the excision of introns. SNRPA1 ensures the fidelity and regulation of mRNA maturation affecting gene expression levels.
The involvement of SNRPA1 in the spliceosome cycle places it within the post-transcriptional modification and gene expression regulation pathways. It is intricately related to other spliceosomal components like U2 snRNP which is essential for the branch point recognition in the splicing process. Together with proteins such as SF3B1 SNRPA1 plays a role in the coordination and timing of splice site recognition and catalysis during the splicing pathway.
SNRPA1's role in mRNA splicing links it to conditions like spinal muscular atrophy (SMA) and some cancers. Spinal muscular atrophy involving survival motor neuron (SMN) protein dysfunction can be influenced by perturbed alternative splicing processes where SNRPA1 contributes. In certain cancers aberrant splicing patterns involving SNRPA1 and other kinases may lead to oncogenic transformations indicating its importance in maintaining splicing accuracy during cell growth and division.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-SNRPA1 antibody [EPR7557] ab128937, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SNRPA1 antibody [EPR7557] (Anti-SNRPA1 antibody [EPR7557] ab128937) at 1/1000 dilution
Lane 1: Huvec cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: Rat liver tissue lysate at 10 µg
Lane 5: Mouse liver tissue lysate at 10 µg
All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
This data was developed using Anti-SNRPA1 antibody [EPR7557] ab128937, the same antibody clone in a different buffer formulation.Anti-SNRPA1 antibody [EPR7557] ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using Anti-SNRPA1 antibody [EPR7557] ab128937, the same antibody clone in a different buffer formulation.Anti-SNRPA1 antibody [EPR7557] ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human squamous lung cell carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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