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AB155026

Anti-SNRPB/SmB antibody

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(4 Publications)

Rabbit Polyclonal SNRPB/SmB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

COD, SNRPB1, SNRPB, Small nuclear ribonucleoprotein-associated proteins B and B', snRNP-B, Sm protein B/B', Sm-B/B', SmB/B'

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SNRPB/SmB antibody (AB155026)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SNRPB/SmB antibody (AB155026)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SNRPB protein at cytoplasm and nucleus using ab155026 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Scale bar= 10µm.

Immunocytochemistry/ Immunofluorescence - Anti-SNRPB/SmB antibody (AB155026)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SNRPB/SmB antibody (AB155026)

Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SNRPB/SmB with ab155026 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.

Western blot - Anti-SNRPB/SmB antibody (AB155026)
  • WB

Unknown

Western blot - Anti-SNRPB/SmB antibody (AB155026)

12% SDS PAGE

All lanes:

Western blot - Anti-SNRPB/SmB antibody (ab155026) at 1/1000 dilution

All lanes:

Hela nuclear lysate at 30 µg

Predicted band size: 25 kDa,46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPB also known as SmB is a core component of the spliceosomal small nuclear ribonucleoproteins (snRNPs). It has a molecular weight of approximately 25 kDa. This protein is widely expressed in human tissues especially in those with high proliferative capacity like the brain and immune system. SNRPB plays an essential role in the splicing of pre-mRNA by binding to it and facilitating its processing into mature mRNA.
Biological function summary

SNRPB functions as part of the spliceosome complex which is responsible for removing introns from pre-mRNA. It assists in assembling and stabilizing the snRNPs required for correct splicing. The protein helps ensure cellular pathways operate smoothly by maintaining RNA integrity which is important for gene regulation and expression. SNRPB with other Sm proteins forms a stable core structure needed for snRNP function within the spliceosome complex.

Pathways

Scientists connect SNRPB to the mRNA splicing and gene expression pathways. In particular it interacts closely with other snRNPs like SNRPD1 and SNRPD2 which are also integral to mRNA processing. The accurate function of these proteins within the pathway ensures that cells produce correct protein variants which are essential for cell survival and adaptation to environmental changes.

Researchers often associate SNRPB with spinal muscular atrophy (SMA) and systemic lupus erythematosus (SLE). In SMA dysfunction in snRNP assembly due to mutations in related proteins like SMN1 can lead to motor neuron degeneration. In SLE autoimmune reactions often target snRNPs including SNRPB leading to chronic inflammation and tissue damage. Understanding these associations helps in the exploration of potential therapeutic targets for these challenging diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing (PubMed : 12975319).
See full target information SNRPB
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111626 PubMed39850359

2025

Alternative splicing of EZH2 regulated by SNRPB mediates hepatocellular carcinoma progression via BMP2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Wang,Weiyi Liu,Chunai Zhan,Yuanyuan Zhang,Xinyu Li,Yaoyun Wang,Mengfei Sheng,Madiha Maqsood,Hang Shen,Anmin Liang,Wei Shao

Oncology letters 27:259 PubMed38646492

2024

Clinical prognostic significance of xeroderma pigmentosum group C and IFN‑γ in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongming Wang,Weiyu Wang,Huaijie Wang,Liya Qin,Meijia Zhang,Yong Zhang,Yubing Wang,Changcheng Hao,Meihua Qu,Gongchao Wang

Open medicine (Warsaw, Poland) 17:1623-1631 PubMed36329787

2022

SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Deng,Xin Li,Wenlei Jiang,Jindan Tang

International journal of endocrinology 2022:9433434 PubMed35242185

2022

lncRNA RUNDC3A-AS1 Regulates Proliferation and Apoptosis of Thyroid Cancer Cells via the miR-151b/SNRPB Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Deng,Jie Wu,Xin Li
View all publications
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