AB59774

Anti-SNRPD1 antibody [3032C3a]

Name: Anti-SNRPD1 antibody [3032C3a] (ab59774) | Abcam
Brand: Abcam Limited
SKU: AB59774
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal SNRPD1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNRPD1.

View Alternative Names

Small nuclear ribonucleoprotein Sm D1, Sm-D1, Sm-D autoantigen, snRNP core protein D1, SNRPD1

1 Images

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Western blot - Anti-SNRPD1 antibody [3032C3a] (AB59774)

Note : The difference between predicted and actual band sizes may be due to the fact that ab59774 was tested against a recombinant protein fragment, and not the full length protein.

All lanes:

Western blot - Anti-SNRPD1 antibody [3032C3a] (ab59774)

All lanes:

immunizing peptide

Predicted band size: 13 kDa

Observed band size: 35 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3032C3a

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Human SNRPD1. The exact immunogen used to generate this antibody is proprietary information.

P62314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Filtered through a 0.22 µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPD1 also known as Sm-D1 or Small Nuclear Ribonucleoprotein D1 is a protein that functions as part of the spliceosome complex. This protein with a molecular mass of approximately 13 kDa contributes to the assembly and function of the spliceosome. SNRPD1 is highly expressed in the nucleus of eukaryotic cells where it interacts with other spliceosomal components to carry out RNA splicing processes.

Biological function summary

SNRPD1 plays a critical role in the splicing of pre-mRNA as part of the small nuclear ribonucleoprotein (snRNP) core complex. It specifically binds to small nuclear RNA (snRNA) molecules aiding in the formation and stabilization of the snRNP structure. Through this activity SNRPD1 cooperates with other snRNPs to facilitate the removal of introns from pre-mRNA a necessary step in the synthesis of mature and functional mRNA transcripts.

Pathways

SNRPD1 engages in the spliceosome pathway which involves the precise removal of non-coding sequences from RNA transcripts. SNRPD1 is closely associated with proteins such as SNRPB and SNRPC which are integral components of the same snRNP complex. Its involvement in the spliceosome pathway highlights its important role in the regulation of gene expression and RNA processing within the cell.

Alterations in SNRPD1 function have been implicated in certain types of cancer including breast cancer by impacting mRNA splicing. In this context SNRPD1 may interact with oncogenic factors and other spliceosome-associated proteins like SF3B1 which also modulate spliceosome activity. Additionally mutations or dysregulation of SNRPD1 have associations with autoimmune conditions such as systemic lupus erythematosus where the immune system may target spliceosome components disrupting normal cellular functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 23333303, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA (PubMed : 23333303).

See full target information SNRPD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2022:6918396 PubMed35909488

2022

hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangwei Jiang,Mingxuan Qu,Lili Kong,Xiaoming Song,Shanhao Jiang

Cell reports 30:1935-1950.e8 PubMed32049022

2020

Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick J Metz,Keith A Ching,Tao Xie,Paulina Delgado Cuenca,Sherry Niessen,John H Tatlock,Kristen Jensen-Pergakes,Brion W Murray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SNRPD1 antibody [3032C3a]

Western blot - Anti-SNRPD1 antibody [3032C3a] (AB59774)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise