AB233115

Anti-SNRPD1 antibody

Name: Anti-SNRPD1 antibody (ab233115) | Abcam
Brand: Abcam Limited
SKU: AB233115
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal SNRPD1 antibody. Suitable for WB, IHC-P and reacts with Rat, Pig, Recombinant fragment - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNRPD1.

View Alternative Names

Small nuclear ribonucleoprotein Sm D1, Sm-D1, Sm-D autoantigen, snRNP core protein D1, SNRPD1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Paraffin-embedded human liver tissue stained for SNRPD1 using ab233115 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Paraffin-embedded human kidney tissue stained for SNRPD1 using ab233115 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Paraffin-embedded human stomach tissue stained for SNRPD1 using ab233115 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Paraffin-embedded human cerebrum tissue stained for SNRPD1 using ab233115 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Paraffin-embedded human prostate tissue stained for SNRPD1 using ab233115 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-SNRPD1 antibody (AB233115)

All lanes:

Western blot - Anti-SNRPD1 antibody (ab233115) at 1 µg/mL

Lane 1:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 3:

Rat testis lysate

Lane 4:

Pig kidney lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-SNRPD1 antibody (AB233115)

All lanes:

Western blot - Anti-SNRPD1 antibody (ab233115) at 2 µg/mL

Lane 1:

Wild type Jurkat cells

Lane 2:

SNRPD1 knockdown Jurkat cells

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-SNRPD1 antibody (AB233115)

All lanes:

Western blot - Anti-SNRPD1 antibody (ab233115) at 1 µg/mL

All lanes:

Recombinant human SNRPD1 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SNRPD1.

P62314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab233115 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPD1 also known as Sm-D1 or Small Nuclear Ribonucleoprotein D1 is a protein that functions as part of the spliceosome complex. This protein with a molecular mass of approximately 13 kDa contributes to the assembly and function of the spliceosome. SNRPD1 is highly expressed in the nucleus of eukaryotic cells where it interacts with other spliceosomal components to carry out RNA splicing processes.

Biological function summary

SNRPD1 plays a critical role in the splicing of pre-mRNA as part of the small nuclear ribonucleoprotein (snRNP) core complex. It specifically binds to small nuclear RNA (snRNA) molecules aiding in the formation and stabilization of the snRNP structure. Through this activity SNRPD1 cooperates with other snRNPs to facilitate the removal of introns from pre-mRNA a necessary step in the synthesis of mature and functional mRNA transcripts.

Pathways

SNRPD1 engages in the spliceosome pathway which involves the precise removal of non-coding sequences from RNA transcripts. SNRPD1 is closely associated with proteins such as SNRPB and SNRPC which are integral components of the same snRNP complex. Its involvement in the spliceosome pathway highlights its important role in the regulation of gene expression and RNA processing within the cell.

Alterations in SNRPD1 function have been implicated in certain types of cancer including breast cancer by impacting mRNA splicing. In this context SNRPD1 may interact with oncogenic factors and other spliceosome-associated proteins like SF3B1 which also modulate spliceosome activity. Additionally mutations or dysregulation of SNRPD1 have associations with autoimmune conditions such as systemic lupus erythematosus where the immune system may target spliceosome components disrupting normal cellular functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 23333303, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA (PubMed : 23333303).

See full target information SNRPD1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer (Dove Medical Press) 16:679-688 PubMed39411513

2024

The Potential Role of SNRPD1 Stabilized by IGF2BP2 in the Progression of Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Liu,Xin Sun,Na Liu,Fangcai Lin

Cells 12: PubMed37998393

2023

LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lubna M Mehdawi,Souvik Ghatak,Payel Chakraborty,Anita Sjölander,Tommy Andersson

Journal of Cancer 13:184-201 PubMed34976182

2022

The diagnostic and prognostic significance of small nuclear ribonucleoprotein Sm D1 aberrantly high expression in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huaxiang Wang,Fengfeng Xu,Lingling Lu,Fang Yang,Xinghua Huang,Lizhi Lv,Huanzhang Hu,Yi Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNRPD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD1 antibody (AB233115)

Western blot - Anti-SNRPD1 antibody (AB233115)

Western blot - Anti-SNRPD1 antibody (AB233115)

Western blot - Anti-SNRPD1 antibody (AB233115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise