Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (AB251254)

This data was developed using ab198296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (AB251254)

This data was developed using ab198296, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells labelling SNRPD2 (red) with purified ab198296 at dilution of 1/150. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

• IP

Supplier Data

Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (AB251254)

This data was developed using ab198296&44; the same antibody clone in a different buffer formulation.

NRPD2 protein was immunoprecipitation from 1mg of MCF-7 (Human breast adenocarcinoma) whole cell lysate with ab198296 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab198296 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1 : Input, MCF-7 (Human breast adenocarcinoma) whole cell lysate, 10ug. Lane 2 : IP of NRPD2 from MCF-7 (Human breast adenocarcinoma) whole cell lysate. Lane 3 : IP using Rabbit monoclonal IgG (ab172730) instead of ab198296 in MCF-7 (Human breast adenocarcinoma) whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SNRPD2 antibody [EPR16762] (<a href='/en-us/products/primary-antibodies/snrpd2-antibody-epr16762-ab198296'>ab198296</a>)

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 5s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (AB251254)

This data was developed using ab198296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling SNRPD2 with ab198296 at 1/600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

Negative control Using PBS instead of primary antbody, secondary ab is Goat Anti-Rabbit IgG H&L (HRP)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (AB251254)

This data was developed using ab198296, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNRPD2 antibody [EPR16762] (<a href='/en-us/products/primary-antibodies/snrpd2-antibody-epr16762-ab198296'>ab198296</a>) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-SNRPD2 antibody [EPR16762] - BSA and Azide free (AB251254)

This data was developed using ab198296, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Lanes 1 - 6:

Western blot - Anti-SNRPD2 antibody [EPR16762] (<a href='/en-us/products/primary-antibodies/snrpd2-antibody-epr16762-ab198296'>ab198296</a>) at 1/2000 dilution

Lane 7:

Western blot - Anti-SNRPD2 antibody [EPR16762] (<a href='/en-us/products/primary-antibodies/snrpd2-antibody-epr16762-ab198296'>ab198296</a>) at 1/20000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

true

Exposure time: 30s