AB249169

Anti-SNRPF antibody [EPR10388] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SNRPF antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PBSCF, SNRPF, Small nuclear ribonucleoprotein F, snRNP-F, Sm protein F, Sm-F, SmF

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SNRPF with ab154870 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SNRPF with ab154870 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal prostate tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154870 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

This data was developed using ab154870, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNRPF antibody [EPR10388] (<a href='/en-us/products/primary-antibodies/snrpf-antibody-epr10388-ab154870'>ab154870</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HL-60 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 10 kDa

false

Unconjugated

Anti-SNRPF antibody [EPR10388]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10388

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249169 is the carrier-free version of ab154870.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SNRPF also known as Small Nuclear Ribonucleoprotein Polypeptide F plays a mechanical role in the spliceosomal complex. It is a component of the U1 U2 U4 U5 and U6 small nuclear ribonucleoproteins (snRNPs) essential in mRNA splicing. This protein has a mass of approximately 9.5 kDa. It is mainly expressed in the nucleus of eukaryotic cells where it participates in RNA processing.

Biological function summary

SNRPF functions as a part of the spliceosome assembly a large ribonucleoprotein complex responsible for pre-mRNA splicing. This process modifies nascent RNA transcripts converting them into mature mRNA for translation. The role of SNRPF in association with other Sm core proteins enables the assembly and stabilization of the snRNPs facilitating the accurate execution of the splicing cycle.

Pathways

The involvement of SNRPF in mRNA processing stands out significantly. It acts within the spliceosome complex playing an important part in the mRNA splicing pathway. This pathway ensures the removal of introns and the proper joining of exons vital for producing functional RNA transcripts. SNRPF interacts with other proteins like SNRPD1 and SNRPD2 forming an intricate network pivotal in the regulation of gene expression.

Defects in SNRPF expression or function have links to neurodegenerative disorders and certain cancers. Misregulation of splicing events potentially caused by altered levels of SNRPF connects to conditions such as spinal muscular atrophy. The protein's interactions with other splicing factors might influence the development of these diseases highlighting the importance of its proper regulation in cellular processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed : 11991638, PubMed : 18984161, PubMed : 19325628, PubMed : 23333303, PubMed : 25555158, PubMed : 26912367, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166, PubMed : 32494006). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770, PubMed : 28781166). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed : 12975319).

See full target information SNRPF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNRPF antibody [EPR10388] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Western blot - Anti-SNRPF antibody [EPR10388] - BSA and Azide free (AB249169)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise