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AB326247

SNv428 Anti-CPT1A antibody [EPR21843-71-1C]

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Rabbit Recombinant Monoclonal CPT1A antibody - conjugated to SNv428. Suitable for Flow Cyt(Intra) and reacts with Human samples.

This is a Beckman Coulter product distributed by Abcam.
1 Images
Flow Cytometry (Intracellular) - SNv428 Anti-CPT1A antibody [EPR21843-71-1C] (AB326247)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-CPT1A antibody [EPR21843-71-1C] (AB326247)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and CPT1A knockout HAP1 stained with ab326247 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab326247) (1x 106 in 100μl at 1 μg/ml (1/200)) for 30min at 22°C.

Unstained control in HAP1 WT cells (black line) and HAP1-CPT1A KO cells (grey line) was also included.

Acquisition of >5000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.

This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

  • Unconjugated

    Anti-CPT1A antibody [EPR21843-71-1C]

  • Carrier free

    Anti-CPT1A antibody [EPR21843-71-1C] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21843-71-1C

Isotype

IgG

Conjugation

SNv428

Excitation/Emission

Ex: 405nm, Em: 428nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This is a Beckman Coulter product distributed by Abcam.

The SuperNova dye included in this product is provided under an intellectual property license from Beckman Coulter Life Sciences. As this product contains the SuperNova dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity).

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA, 0.02% Pluronic F-68
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed : 11350182, PubMed : 14517221, PubMed : 16651524, PubMed : 9691089). Also possesses a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity (PubMed : 29425493). Plays an important role in hepatic triglyceride metabolism (By similarity). Also plays a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression (By similarity). Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation (PubMed : 38016475). Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).
See full target information CPT1A

Alternative Names

CPT1, CPT1A, CPT1-L, Carnitine palmitoyltransferase 1A, Succinyltransferase CPT1A, CPT I, CPTI-L
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com