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AB326119

SNv428 Anti-Hexokinase 1 antibody [EPR10134(B)]

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Rabbit Recombinant Monoclonal Hexokinase 1 antibody - conjugated to SNv428. Suitable for Flow Cyt (Intra) and reacts with Human samples.

This is a Beckman Coulter product distributed by Abcam

View Alternative Names

Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1

2 Images
Flow Cytometry (Intracellular) - SNv428 Anti-Hexokinase 1 antibody [EPR10134(B)] (AB326119)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Hexokinase 1 antibody [EPR10134(B)] (AB326119)

Flow cytometry overlay histogram showing wild-type HEK293T (green line) and HK1 knockout HEK293T stained with ab326119 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab326119) (1x 106 in 100µl at 0.2 µg/ml (1/1000)) for 30min at 22°C.Unstained control in HEK293T WT cells (black line) and HEK293T-HK1 KO cells (grey line) was also included. Acquisition of >5000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter. This antibody gave a positive signal in wild-type HEK293T fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

Flow Cytometry (Intracellular) - SNv428 Anti-Hexokinase 1 antibody [EPR10134(B)] (AB326119)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Hexokinase 1 antibody [EPR10134(B)] (AB326119)

Flow cytometry scatter plots showing human peripheral mononuclear cells (PBMCs) stained with ab326119 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 µg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326119) (1x 106 in 100µl at 0.2 µg/ml (1/1000)) for 30min on ice. The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

  • Unconjugated

    Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • Mouse version

    Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial OMM – Mouse IgG1 (Chimeric)

  • Mouse version

    Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free

  • Carrier free

    Anti-Hexokinase 1 antibody [EPR10134(B)] - BSA and Azide free

  • 660 APC

    APC Anti-Hexokinase 1 - Mitochondrial Outer Membrane Marker antibody [EPR10134(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker

  • HRP

    HRP Anti-Hexokinase 1 - Mitochondrial Outer Membrane Marker antibody [EPR10134(B)]

  • 578 PE

    PE Anti-Hexokinase 1 - Mitochondrial Outer Membrane Marker antibody [EPR10134(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10134(B)

Isotype

IgG

Conjugation

SNv428

Excitation/Emission

Ex: 405nm, Em: 428nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p>Use of the SuperNova Staining Buffer (<a href='/en-us/products/buffers/supernova-staining-buffer-ab327075'>ab327075</a>) is recommended when your multicolor staining cocktail contains more than one SuperNova/polymer dye antibody conjugate.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This is a Beckman Coulter product distributed by Abcam.

The SuperNova dye included in this product is provided under an intellectual property license from Beckman Coulter Life Sciences. As this product contains the SuperNova dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity).

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA, 0.02% Pluronic F-68
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed : 1637300, PubMed : 25316723, PubMed : 27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed : 27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed : 27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed : 27374331).
See full target information HK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com