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AB326186

SNv428 Anti-OGDH antibody [EPR27181-78]

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Rabbit Recombinant Monoclonal OGDH antibody - conjugated to SNv428.
Suitable for Flow Cyt(Intra) and reacts with Human samples.

This is a Beckman Coulter product distributed by Abcam
1 Images
Flow Cytometry (Intracellular) - SNv428 Anti-OGDH antibody [EPR27181-78] (AB326186)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-OGDH antibody [EPR27181-78] (AB326186)

Flow cytometry scatter plots showing untreated human peripheral mononuclear cells (PBMCs) (top) and PBMCs activated with 5 μg/ml PHA for 18h (bottom), either stained with ab326186 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 μg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326186) (1x 106 in 100μl at 1 μg/ml (1/200)) for 30min on ice. The cells were simultaneously stained with CD3. Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27181-78

Isotype

IgG

Conjugation

SNv428

Excitation/Emission

Ex: 405nm, Em: 428nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This is a Beckman Coulter product distributed by Abcam.

The SuperNova dye included in this product is provided under an intellectual property license from Beckman Coulter Life Sciences. As this product contains the SuperNova dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity).

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA, 0.02% Pluronic F-68
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OGDH also known as alpha-ketoglutarate dehydrogenase or a-ketoglutarate dehydrogenase is an enzyme that plays a significant role in metabolism. It catalyzes the conversion of alpha-ketoglutarate into succinyl-CoA and CO2 in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. The enzyme functions as a part of a multi-enzyme complex and has a molecular mass of approximately 1 MDa. OGDH is expressed in various tissues with higher levels typically found in the mitochondria of cells reflecting its role in cellular respiration.
Biological function summary

OGDH is critical for energy production by enabling the TCA cycle to proceed. It is a component of the alpha-ketoglutarate dehydrogenase complex consisting of multiple subunits which include E1 (OGDH itself) E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase). This complex functions to ensure the proper oxidation of metabolites important for maintaining cellular energy homeostasis. Proper regulation of OGDH activity impacts several metabolic pathways influencing cellular glucose and lipid metabolism.

Pathways

OGDH is integrally involved in the TCA cycle a central metabolic pathway that connects carbohydrate fat and protein metabolism. Alongside related enzymes like pyruvate dehydrogenase (PDH) and isocitrate dehydrogenase (IDH) OGDH contributes to the generation of NADH and FADH2 essential carriers in the electron transport chain. The interplay between these enzymes ensures the smooth functioning of aerobic respiration linking glycolysis and oxidative phosphorylation.

OGDH has links to neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Abnormal activity of OGDH can lead to mitochondrial dysfunction a common feature observed in these disorders. The protein connects to pathways involving PDH dysfunction contributing to altered metabolic states seen in these conditions. Research continues to explore the potential of targeting OGDH in therapeutic strategies aimed at improving mitochondrial health and energy metabolism in affected patients.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC) (PubMed : 24495017, PubMed : 25210035, PubMed : 28435050). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC (PubMed : 24495017, PubMed : 25210035, PubMed : 28435050). Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST) (PubMed : 24495017, PubMed : 25210035, PubMed : 28435050, PubMed : 35272141). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids (PubMed : 25210035). Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate (PubMed : 28435050). Can also convert 2-keto-4-hydroxyglutarate (KHG) and CoA into malyl-CoA (By similarity). Mainly active in the mitochondrion (PubMed : 29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones : associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed : 29211711).
See full target information 2-oxoglutarate dehydrogenase, mitochondrial

Alternative Names

2-oxoglutarate dehydrogenase complex component E1, E1o, HsOGDH, OGDC-E1, OGDH-E1, Alpha-ketoglutarate dehydrogenase, Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase, Alpha-KGDH-E1, OGDH
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Product promise

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