SNv428 Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB326225)

Flow cytometry scatter plots showing untreated human peripheral mononuclear cells (PBMCs) (top) and PBMCs activated with 5 μg/ml PHA for 18h (bottom), either stained with ab326225 (right) or FMO control (left). The cells were fixed and permeabilised using BD CytoFix/CytoPerm™ (20 min). The cells were incubated in 1x PBS containing 10 μg/ml human IgG, 10% normal goat serum and BD Pharmingen™ MonoBlock™ Leukocyte Staining Buffer (1/10) to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab326225) (1x 10⁶ 100μl at 5 μg/ml (1/40)) for 30min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.

This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - SNv428 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB326225)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and PRDX2 knockout HAP1 stained with ab326225 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab326225) (1x 10⁶ in 100μl at 1 μg/ml (1/200)) for 30min at 22°C.

Unstained control in HAP1 WT cells (black line) and HAP1-PRDX2 KO cells (grey line) was also included.

Acquisition of >5000 events were collected using a 100 mW Violet laser (405nm) and 450/45 bandpass filter.

This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.