AB307513

Anti-SNX17 antibody [EPR26025-45]

20ul selling size
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SNX17 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA0064, SNX17, Sorting nexin-17

4 Images

Supplier Data

Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (AB307513)

SNX17 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab307513 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307513 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2 : ab307513 IP in HeLa whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307513 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 67 seconds

All lanes:

Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (ab307513) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2:

HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 53 kDa

false

Exposure time: 67s

Supplier Data

Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (AB307513)

SNX17 was immunoprecipitated from 0.35 mg RAW264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with ab307513 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307513 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug
Lane 2 : ab307513 IP in RAW264.7 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307513 in RAW264.7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 67 seconds

All lanes:

Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (ab307513) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg

Lane 2:

RAW264.7 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 53 kDa

false

Exposure time: 67s

Supplier Data

Western blot - Anti-SNX17 antibody [EPR26025-45] (AB307513)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 180seconds

All lanes:

Western blot - Anti-SNX17 antibody [EPR26025-45] (ab307513) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20 μg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 20 μg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 5:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

Lane 6:

RAW264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

Lane 7:

MEF (mouse embryonic fibroblast ) whole cell lysate 20 μg

Lane 8:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

Lane 9:

C6 (rat glial tumor glial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,53 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-SNX17 antibody [EPR26025-45] (AB307513)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe Lanes 2-5 were developed using a high sensitivity ECL substrate. Exposure time :

All lanes:

Western blot - Anti-SNX17 antibody [EPR26025-45] (ab307513) at 1/1000 dilution

Lane 1:

Human heart tissue lysate 20 μg

Lane 2:

Mouse fetal brain tissue lysate 20 μg

Lane 3:

Mouse heart tissue lysate 20 μg

Lane 4:

Rat fetal brain tissue lysate 20 μg

Lane 5:

Rat heart tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,53 kDa

true

Exposure time: 37s

Carrier free

Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26025-45

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/32", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels (PubMed : 15121882, PubMed : 15769472). Binds to NPxY sequences in the cytoplasmic tails of target cargos (PubMed : 21512128). Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits (PubMed : 22492727, PubMed : 28892079). Also required for maintenance of normal cell surface levels of APP and LRP1 (PubMed : 16712798, PubMed : 19005208). Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) (PubMed : 16712798).

See full target information SNX17

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8794 PubMed41038817

2025

Identification of a RAB32-LRMDA-Commander membrane trafficking complex reveals the molecular mechanism of human oculocutaneous albinism type 7.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeka Butkovič,Michael D Healy,Cecilia de Heus,Alexander P Walker,Wyatt Beyers,Kerrie E McNally,Philip A Lewis,Kate J Heesom,Nalan Liv,Judith Klumperman,Santiago Di Pietro,Brett M Collins,Peter J Cullen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SNX17 antibody [EPR26025-45]

Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (AB307513)

Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (AB307513)

Western blot - Anti-SNX17 antibody [EPR26025-45] (AB307513)

Western blot - Anti-SNX17 antibody [EPR26025-45] (AB307513)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise