Rabbit Recombinant Monoclonal SNX17 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Images
![Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--immunoprecipitation-img406415.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514)")
![Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--immunoprecipitation-img406416.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514)")
![Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--western-blot-img405695.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514)")
![Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--western-blot-img405694.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (AB307514)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Expected | Not recommended | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels (PubMed:15121882, PubMed:15769472). Binds to NPxY sequences in the cytoplasmic tails of target cargos (PubMed:21512128). Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits (PubMed:22492727, PubMed:28892079). Also required for maintenance of normal cell surface levels of APP and LRP1 (PubMed:16712798, PubMed:19005208). Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) (PubMed:16712798).
Alternative names
KIAA0064, SNX17, Sorting nexin-17
Recommended products
Rabbit Recombinant Monoclonal SNX17 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26025-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--immunoprecipitation-img406415.jpg "Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)")
Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)
This data was developed using Anti-SNX17 antibody [EPR26025-45] ab307513, the same antibody clone in a different buffer formulation.
SNX17 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with Anti-SNX17 antibody [EPR26025-45] ab307513 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SNX17 antibody [EPR26025-45] ab307513 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB307513 IP in HeLa whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SNX17 antibody [EPR26025-45] ab307513 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 67 secondsAll lanes: Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (Anti-SNX17 antibody [EPR26025-45] ab307513) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: HeLa whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 53 kDa
Exposure time: 67s
![Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--immunoprecipitation-img406416.jpg "Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)")
Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)
This data was developed using Anti-SNX17 antibody [EPR26025-45] ab307513, the same antibody clone in a different buffer formulation.
SNX17 was immunoprecipitated from 0.35 mg RAW264.7 (mouse ABelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug with Anti-SNX17 antibody [EPR26025-45] ab307513 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SNX17 antibody [EPR26025-45] ab307513 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: RAW264.7 (mouse ABelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 ug
Lane 2: ABAB307513 IP in RAW264.7 whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SNX17 antibody [EPR26025-45] ab307513 in RAW264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 67 secondsAll lanes: Immunoprecipitation - Anti-SNX17 antibody [EPR26025-45] (Anti-SNX17 antibody [EPR26025-45] ab307513) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg
Lane 2: RAW264.7 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 53 kDa
Exposure time: 67s
![Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--western-blot-img405695.jpg "Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)")
Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)
This data was developed using 307513, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The Lanes 2-5 were developed using a high sensitivity ECL substrate.
Exposure time:All lanes: Western blot - Anti-SNX17 antibody [EPR26025-45] (Anti-SNX17 antibody [EPR26025-45] ab307513) at 1/1000 dilution
Lane 1: Human heart tissue lysate 20 μg
Lane 2: Mouse fetal brain tissue lysate 20 μg
Lane 3: Mouse heart tissue lysate 20 μg
Lane 4: Rat fetal brain tissue lysate 20 μg
Lane 5: Rat heart tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 50 kDa, 53 kDa
Exposure time: 37s
![Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514), expandable thumbnail](https://content.abcam.com/products/images/snx17-antibody-epr26025-45-bsa-and-azide-free-ab307514--western-blot-img405694.jpg "Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)")
Western blot - Anti-SNX17 antibody [EPR26025-45] - BSA and Azide free (ab307514)
This data was developed using 307513, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration:
180seconds
Exposure time:All lanes: Western blot - Anti-SNX17 antibody [EPR26025-45] (Anti-SNX17 antibody [EPR26025-45] ab307513) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20 μg
Lane 3: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 20 μg
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 5: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg
Lane 6: RAW264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg
Lane 7: MEF (mouse embryonic fibroblast ) whole cell lysate 20 μg
Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg
Lane 9: C6 (rat glial tumor glial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50 kDa, 53 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com