Rabbit Recombinant Monoclonal SNX27 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425562.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898)")
![Immunoprecipitation - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--immunoprecipitation-img425642.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898)")
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425563.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898)")
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898)")
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425561.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (AB315898)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | Flow Cyt (Intra) | IHC-P | IHC-Fr | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Expected | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.
Alternative names
Kiaa0488, Snx27, Sorting nexin-27
Recommended products
Rabbit Recombinant Monoclonal SNX27 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28130-16
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315898 is the carrier-free version of Anti-SNX27 antibody [EPR28130-16] ab315897.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425562.jpg "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)
This data was developed using Anti-SNX27 antibody [EPR28130-16] ab315897, the same antibody clone in a different buffer formulation.
This blot in lane 5 was developed using a high sensitivity ECL substrate.
Lanes 1-4 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lane 5 is applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SNX27 antibody [EPR28130-16] (Anti-SNX27 antibody [EPR28130-16] ab315897) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse thymus tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse spinal cord tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryLanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 5: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 60 kDa, 36 kDa
Exposure time: 180s
![Immunoprecipitation - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--immunoprecipitation-img425642.jpg "Immunoprecipitation - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Immunoprecipitation - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)
This data was developed using Anti-SNX27 antibody [EPR28130-16] ab315897, the same antibody clone in a different buffer formulation.
SNX27 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-SNX27 antibody [EPR28130-16] ab315897 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-SNX27 antibody [EPR28130-16] ab315897 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: Anti-SNX27 antibody [EPR28130-16] ab315897 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SNX27 antibody [EPR28130-16] ab315897 in NIH/3T3 whole cell lysateAll lanes: Immunoprecipitation - Anti-SNX27 antibody [EPR28130-16] (Anti-SNX27 antibody [EPR28130-16] ab315897) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425563.jpg "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)
This data was developed using Anti-SNX27 antibody [EPR28130-16] ab315897, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SNX27 antibody [EPR28130-16] (Anti-SNX27 antibody [EPR28130-16] ab315897) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg with 5% NFDM/TBST
Lane 6: Rat spinal cord tissue lysate at 40 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa
Exposure time: 180s
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425560.jpg "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)
This data was developed using Anti-SNX27 antibody [EPR28130-16] ab315897, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SNX27 antibody [EPR28130-16] (Anti-SNX27 antibody [EPR28130-16] ab315897) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: NIH/3T3 transfected with siRNA specifically targeting SNX27 whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa, 36 kDa
Exposure time: 26s
![Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--western-blot-img425561.jpg "Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Western blot - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)
This data was developed using Anti-SNX27 antibody [EPR28130-16] ab315897, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SNX27 antibody [EPR28130-16] (Anti-SNX27 antibody [EPR28130-16] ab315897) at 1/1000 dilution
Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: CTLL-2 (mouse T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 60 kDa, 36 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--flow-cytometry-intracellular-img425652.jpg "Flow Cytometry (Intracellular) - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Flow Cytometry (Intracellular) - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)
This data was developed using Anti-SNX27 antibody [EPR28130-16] ab315897, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling SNX27 with Anti-SNX27 antibody [EPR28130-16] ab315897 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898), expandable thumbnail](https://content.abcam.com/products/images/snx27-antibody-epr28130-16-bsa-and-azide-free-ab315898--flow-cytometry-intracellular-img425652.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SNX27 antibody [EPR28130-16] - BSA and Azide free (ab315898)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com