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AB121600

Anti-SNX30 antibody

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(3 Publications )

Rabbit Polyclonal SNX30 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Sorting nexin-30 aa 1-150.

View Alternative Names

Sorting nexin-30, SNX30

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX30 antibody (AB121600)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX30 antibody (AB121600)

ab121600 at 1/125 dilution staining SNX30 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
Note : strong cytoplasmic positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-SNX30 antibody (AB121600)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SNX30 antibody (AB121600)

Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab121600 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-SNX30 antibody (AB121600)
  • WB

Unknown

Western blot - Anti-SNX30 antibody (AB121600)

All lanes:

Western blot - Anti-SNX30 antibody (ab121600) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

Human Plasma

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 50 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Sorting nexin-30 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q5VWJ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the regulation of endocytosis and in several stages of intracellular trafficking (PubMed : 32513819). Together with SNX4, involved in autophagosome assembly (PubMed : 32513819).
See full target information Sorting nexin-30
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 20:51 PubMed39780196

2025

SNX30 inhibits lung adenocarcinoma cell proliferation and induces cell ferroptosis through regulating SETDB1.

Applications

Unspecified application

Species

Unspecified reactive species

Xinjie Fan,Qichu Zhu,Chengzhuo Du,Jinlai Chen,Yingming Su

Journal of cell science 133: PubMed32513819

2020

A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Zuriñe Antón,Virginie M S Betin,Boris Simonetti,Colin J Traer,Naomi Attar,Peter J Cullen,Jon D Lane

The Journal of cell biology 216:3695-3712 PubMed28935633

2017

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

Applications

Unspecified application

Species

Unspecified reactive species

Boris Simonetti,Chris M Danson,Kate J Heesom,Peter J Cullen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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