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AB236147

Anti-SNX4 antibody [EPR16954] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SNX4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Sorting nexin-4, SNX4

3 Images
Flow Cytometry (Intracellular) - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)

Intracellular Flow Cytometry analysis of HeLa cells labelling SNX4 (red) with purified ab198504 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198504).

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)
  • IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)

Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : 293 whole cell lysate 10µg.

Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198504).

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (<a href='/en-us/products/primary-antibodies/snx4-antibody-epr16954-c-terminal-ab198504'>ab198504</a>)

Predicted band size: 52 kDa

true

Exposure time: 2min

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)
  • IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - BSA and Azide free (AB236147)

Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : PC12 whole cell lysate 10µg.

Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198504).

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (<a href='/en-us/products/primary-antibodies/snx4-antibody-epr16954-c-terminal-ab198504'>ab198504</a>)

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16954

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab236147 is the carrier-free version of ab198504.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the regulation of endocytosis and in several stages of intracellular trafficking (PubMed : 12668730, PubMed : 17994011, PubMed : 32513819, PubMed : 33468622). Plays a role in recycling endocytosed transferrin receptor and prevent its degradation (PubMed : 17994011). Involved in autophagosome assembly by regulating trafficking and recycling of phospholipid scramblase ATG9A (PubMed : 32513819, PubMed : 33468622).
See full target information SNX4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:105667 PubMed36624844

2023

EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Tang,Wei Wei,John M Snowball,Ernesto S Nakayasu,Sheila M Bell,Charles Ansong,Xinhua Lin,Jeffrey A Whitsett
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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