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AB198504

Anti-SNX4 antibody [EPR16954] - C-terminal

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(4 Publications)

Rabbit Recombinant Monoclonal SNX4 antibody. C-terminal. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

View Alternative Names

Sorting nexin-4, SNX4

6 Images
Flow Cytometry (Intracellular) - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Intracellular Flow Cytometry analysis of HeLa cells labelling SNX4 (red) with purified ab198504 at dilution of 1/60. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)
  • IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : 293 whole cell lysate 10μg.

Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

Predicted band size: 52 kDa

true

Exposure time: 2min

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)
  • IP

Supplier Data

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

Lane 1 (Input) : PC12 whole cell lysate 10μg.

Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.

Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

Predicted band size: 52 kDa

false

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)
  • WB

Supplier Data

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/5000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 30s

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)
  • WB

Supplier Data

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/10000 dilution

All lanes:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 1min

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)
  • WB

Supplier Data

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (AB198504)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/1000 dilution

Lane 1:

C6 whole cell lysate at 10 µg

Lane 2:

RAW 264.7 whole cell lysate at 10 µg

Lane 3:

PC12 whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 15s

  • Carrier free

    Anti-SNX4 antibody [EPR16954] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16954

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the regulation of endocytosis and in several stages of intracellular trafficking (PubMed : 12668730, PubMed : 17994011, PubMed : 32513819, PubMed : 33468622). Plays a role in recycling endocytosed transferrin receptor and prevent its degradation (PubMed : 17994011). Involved in autophagosome assembly by regulating trafficking and recycling of phospholipid scramblase ATG9A (PubMed : 32513819, PubMed : 33468622).
See full target information SNX4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eNeuro 9: PubMed35027445

2022

Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function.

Applications

Unspecified application

Species

Unspecified reactive species

Anddre Osmar Valdivia,Sanjoy K Bhattacharya

Journal of cell science 133: PubMed32513819

2020

A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Zuriñe Antón,Virginie M S Betin,Boris Simonetti,Colin J Traer,Naomi Attar,Peter J Cullen,Jon D Lane

American journal of physiology. Renal physiology 317:F375-F387 PubMed31141395

2019

Involvement of PDZ-SAP97 interactions in regulating AQP2 translocation in response to vasopressin in LLC-PK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed M Nooh,Ajay Kale,Suleiman W Bahouth

The Journal of cell biology 216:3695-3712 PubMed28935633

2017

Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.

Applications

Unspecified application

Species

Unspecified reactive species

Boris Simonetti,Chris M Danson,Kate J Heesom,Peter J Cullen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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