Rabbit Recombinant Monoclonal SNX5 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Images
![Flow Cytometry - Anti-SNX5 antibody [EPR14358] (AB180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--flow-cytometry-img180768.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-SNX5 antibody [EPR14358] (AB180520)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (AB180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168271.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (AB180520)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (AB180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172245.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (AB180520)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (AB180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168647.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (AB180520)")
![Western blot - Anti-SNX5 antibody [EPR14358] (AB180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--western-blot-img175886.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX5 antibody [EPR14358] (AB180520)")
Publications
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- Nature structural & molecular biology 30:958-9692023Architecture of the ESCPE-1 membrane coat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Lopez-Robles,Stefano Scaramuzza,Elsa N Astorga-Simon,Morié Ishida,Chad D Williamson,Soledad Baños-Mateos,David Gil-Carton,Miguel Romero-Durana,Ander Vidaurrazaga,Juan Fernandez-Recio,Adriana L Rojas,Juan S Bonifacino,Daniel Castaño-Díez,Aitor HierroPubMed 37322239
- BMC molecular and cell biology 23:592022Overexpression of lncRNA HOXA-AS2 promotes the progression of oral squamous cell carcinoma by mediating SNX5 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhangyi LiPubMed 36528556
- Nature communications 13:46202022FERARI and cargo adaptors coordinate cargo flow through sorting endosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJachen A Solinger,Harun-Or Rashid,Anne SpangPubMed 35941155
- Proceedings of the National Academy of Sciences of the United States of America 119:e22019801192022ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoris Simonetti,James L Daly,Lorena Simón-Gracia,Katja Klein,Saroja Weeratunga,Carlos Antón-Plágaro,Allan Tobi,Lorna Hodgson,Philip A Lewis,Kate J Heesom,Deborah K Shoemark,Andrew D Davidson,Brett M Collins,Tambet Teesalu,Yohei Yamauchi,Peter J CullenPubMed 35696571
- The Journal of cell biology 221:2022SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongfei Xu,Fei Chang,Shweta Jain,Bradley Austin Heller,Xu Han,Yongjian Liu,Robert H EdwardsPubMed 35426896
- FEBS letters 595:2558-25692021Sorting nexin 6 interacts with Cullin3 and regulates programmed death ligand 1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChinmoy Ghosh,Yanli Xing,Suhua Li,Rosalie G Hoyle,Ming Sun,Jiong Li,Yue SunPubMed 34510437
- Journal of virology 94:2020Porcine Reproductive and Respiratory Syndrome Virus Utilizes Viral Apoptotic Mimicry as an Alternative Pathway To Infect Host Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wei et. al.PubMed 32522856
- Journal of cell science 133:2020Acute inactivation of retromer and ESCPE-1 leads to time-resolved defects in endosomal cargo sorting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley J Evans et. al.PubMed 32747499
- Autophagy :1-172020Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Han et. al.PubMed 32397873
- Cancer cell international 19:2542019Extracellular and macropinocytosis internalized ATP work together to induce epithelial-mesenchymal transition and other early metastatic activities in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanyang Cao et. al.PubMed 31582910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Tested | Expected | Not recommended | Expected | Tested |
| Rat | Tested | Expected | Not recommended | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/50. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/500 | Notes For unpurified use at 1/50. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes For unpurified use at 1/50. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes For unpurified use at 1/100. |
Species Rat | Dilution info 1/50 | Notes For unpurified use at 1/100. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) (PubMed:15561769). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity (PubMed:23085988). Involved in retrograde transport of lysosomal enzyme receptor IGF2R (PubMed:17148574, PubMed:18596235). May function as link between endosomal transport vesicles and dynactin (Probable). Plays a role in the internalization of EGFR after EGF stimulation (Probable). Involved in EGFR endosomal sorting and degradation; the function involves PIP5K1C isoform 3 and is retromer-independent (PubMed:23602387). Together with PIP5K1C isoform 3 facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles (ILVs) of the multivesicular body for subsequent lysosomal degradation (Probable). Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation (PubMed:24610942). Plays a role in macropinocytosis (PubMed:18854019, PubMed:21048941).
Alternative names
Sorting nexin-5, SNX5
Recommended products
Rabbit Recombinant Monoclonal SNX5 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14358
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Flow Cytometry - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--flow-cytometry-img180768.jpg "Flow Cytometry - Anti-SNX5 antibody [EPR14358] (ab180520)")
Flow Cytometry - Anti-SNX5 antibody [EPR14358] (ab180520)
Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling SNX5 with ab180520 at 1/50 dilution (10 μg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168271.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labelling SNX5 with purified ab180520 at 1/500 dilution (0.99 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172245.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labelling SNX5 with purified ab180520 at 1/500 dilution (0.99 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168647.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue sections labelling SNX5 with purified ab180520 at 1/500 dilution (0.99 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Western blot - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--western-blot-img175886.jpg "Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)")
Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
All lanes: Western blot - Anti-SNX5 antibody [EPR14358] (ab180520) at 1/1000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 2: Mouse spleen lysate at 20 µg
Lane 3: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunocytochemistry-immunofluorescence-img174517.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)")
Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)
Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling SNX5 with purified ab180520 at 1/50 dilution (9.9 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--western-blot-img165362.jpg "Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)")
Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
All lanes: Western blot - Anti-SNX5 antibody [EPR14358] (ab180520) at 1/1000 dilution
All lanes: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--immunocytochemistry-immunofluorescence-img174517.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)")
![Western blot - Anti-SNX5 antibody [EPR14358] (ab180520), expandable thumbnail](https://content.abcam.com/products/images/snx5-antibody-epr14358-ab180520--western-blot-img165362.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)")
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