Anti-SNX8 antibody [OTI3C4]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SNX8 antibody [OTI3C4] (AB119388)

Flow Cytometry (Intracellular) analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling SNX8 with ab119388 at a 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388)

Paraffin-embedded human breast adenocarcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388)

Paraffin-embedded human liver tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388)

Paraffin-embedded human tonsil tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388)

Paraffin-embedded human kidney tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388)

Paraffin-embedded human bladder carcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• WB

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Western blot - Anti-SNX8 antibody [OTI3C4] (AB119388)

All lanes:

Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 10 µg

Lane 2:

A549 (Human lung carcinoma cell line) extracts at 10 µg

Lane 3:

COS-7 (African green monkey kidney fibroblast-like cell line) extracts at 10 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) extracts at 10 µg

Lane 5:

MCF7 (Human breast adenocarcinoma cell line) extracts at 10 µg

Predicted band size: 53 kDa

false

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (AB119388)

pCMV6-ENTRY SNX8 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for SNX8 using ab119388 at a 1/100 dilution in ICC/IF.

• WB

Supplier Data

Western blot - Anti-SNX8 antibody [OTI3C4] (AB119388)

All lanes:

Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA at 5 µg

Predicted band size: 46 kDa,47 kDa,53 kDa,65 kDa

false