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Mouse Monoclonal SNX8 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, African green monkey, Transfected cell line samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNX8.

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Images

Western blot - Anti-SNX8 antibody [OTI3C4] (AB119388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (AB119388), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SNX8 antibody [OTI3C4] (AB119388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (AB119388), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human SNX8. Database link Q9Y5X2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
African green monkey
Expected
Tested
Expected
Expected
Transfected cell line
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Target data

Function

May be involved in several stages of intracellular trafficking. May play a role in intracellular protein transport from early endosomes to the trans-Golgi network.

Alternative names

Sorting nexin-8, SNX8

Recommended products

Mouse Monoclonal SNX8 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, African green monkey, Transfected cell line samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNX8.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human SNX8. Database link Q9Y5X2
Clone number
OTI3C4
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Clone OTI3C4 (formerly 3C4).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)

    All lanes: Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 10 µg

    Lane 2: A549 (Human lung carcinoma cell line) extracts at 10 µg

    Lane 3: COS-7 (African green monkey kidney fibroblast-like cell line) extracts at 10 µg

    Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) extracts at 10 µg

    Lane 5: MCF7 (Human breast adenocarcinoma cell line) extracts at 10 µg

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human breast adenocarcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SNX8 antibody [OTI3C4] (ab119388)

    pCMV6-ENTRY SNX8 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for SNX8 using ab119388 at a 1/100 dilution in ICC/IF.

  • Flow Cytometry (Intracellular) - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Flow Cytometry (Intracellular) analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling SNX8 with ab119388 at a 1/100 dilution (red) compared to a non-specific negative control antibody (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human kidney tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human liver tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human bladder carcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)

    Paraffin-embedded human tonsil tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388), expandable thumbnail

    Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388)

    All lanes: Western blot - Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA at 5 µg

    Predicted band size: 46 kDa, 47 kDa, 53 kDa, 65 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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