AB9870

Anti-SOCS1 antibody

Name: Anti-SOCS1 antibody (ab9870) | Abcam
Brand: Abcam Limited
SKU: AB9870
Rating: 5 (4 reviews)

(4 Reviews)

(44 Publications)

Goat Polyclonal SOCS1 antibody. Suitable for IHC-P, ICC, Flow Cyt, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat, Pig samples. Cited in 44 publications. Immunogen corresponding to Synthetic Peptide within Human SOCS1 aa 150 to C-terminus.

View Alternative Names

SSI1, TIP3, SOCS1, Suppressor of cytokine signaling 1, SOCS-1, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3, JAB, SSI-1, TIP-3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (AB9870)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (AB9870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Human Kidney with ab9870 (8 μg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (AB9870)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (AB9870)

Flow cytometric analysis of HEK293 cells with ab9870 (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supplier Data

Immunocytochemistry - Anti-SOCS1 antibody (AB9870)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation with ab9870 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (AB9870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Mouse Kidney with ab9870 (8 μg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Pig, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, Flow Cyt, ICC

applications

Immunogen

Synthetic Peptide within Human SOCS1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15524

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-3 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/goat-igg-polyclonal-isotype-control-ab37373'>ab37373</a> - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-3 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential negative regulator of type I and type II interferon (IFN) signaling, as well as that of other cytokines, including IL2, IL4, IL6 and leukemia inhibitory factor (LIF) (PubMed : 32499645, PubMed : 33087723). Downregulates cytokine signaling by inhibiting the JAK/STAT signaling pathway. Acts by binding to JAK proteins and to IFNGR1 and inhibiting their kinase activity. In vitro, suppresses Tec protein-tyrosine activity (PubMed : 9341160). Regulates IFN-gamma (IFNG)-mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 11278610, PubMed : 11313480).

See full target information SOCS1

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 24:80 PubMed40091029

2025

IL-4 mediated TAP2 downregulation is a dominant and reversible mechanism of immune evasion and immunotherapy resistance in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kishu Ranjan,Barani Kumar Rajendran,Imad Ud Deen,Adrien Costantini,Miguel Lopez de Rodas,Shruti S Desai,Frankie Scallo,Nicole Gianino,Soldano Ferrone,Kurt A Schalper

Future science OA 11:2463852 PubMed39967065

2025

Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Wu,Yixia Zhu,Shun Zheng,Ding Mingxing

European journal of immunology 54:e2350700 PubMed38039407

2023

Zfp36l1 establishes the high-affinity CD8 T-cell response by directly linking TCR affinity to cytokine sensing.

Applications

Unspecified application

Species

Unspecified reactive species

Georg Petkau,Twm J Mitchell,Marian Jones Evans,Louise Matheson,Fiamma Salerno,Martin Turner

World journal of gastroenterology 29:5226-5239 PubMed37901446

2023

Curcumin alleviated dextran sulfate sodium-induced colitis by recovering memory Th/Tfh subset balance.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Xin Zheng,Kai-En Guo,Jia-Qi Huang,Miao-Hua Liu,Bai-Ling Deng,Duan-Yong Liu,Bu-Gao Zhou,Wen Zhou,You-Bao Zhong,Hai-Mei Zhao

International journal of molecular sciences 24: PubMed37685986

2023

Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Ismael Bermejo-Álvarez,Sandra Pérez-Baos,Paula Gratal,Juan Pablo Medina,Raquel Largo,Gabriel Herrero-Beaumont,Aránzazu Mediero

Experimental & molecular medicine 55:831-843 PubMed37009803

2023

LncRNA KIAA0087 suppresses the progression of osteosarcoma by mediating the SOCS1/JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haoli Gong,Ye Tao,Sheng Xiao,Xin Li,Ke Fang,Jie Wen,Pan He,Ming Zeng

Nature communications 13:7500 PubMed36473863

2022

Extracellular traps from activated vascular smooth muscle cells drive the progression of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhai,Shiyu Gong,Peipei Luan,Yefei Shi,Wenxin Kou,Yanxi Zeng,Jiayun Shi,Guanye Yu,Jiayun Hou,Qing Yu,Weixia Jian,Jianhui Zhuang,Mark W Feinberg,Wenhui Peng

International journal of immunopathology and pharmacology 36:3946320221133018 PubMed36214175

2022

Syk promotes phagocytosis by inducing reactive oxygen species generation and suppressing SOCS1 in macrophage-mediated inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Su Yi,Han Gyung Kim,Ji Hye Kim,Woo Seok Yang,Eunji Kim,Jae Gwang Park,Nur Aziz,Narayanan Parameswaran,Jae Youl Cho

Frontiers in immunology 13:934084 PubMed35844489

2022

Effect of Polysaccharide on M1 Polarization Induced by Autophagy Degradation of SOCS1/2 Proteins in 3D4/21 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Li,Xufang Chen,Meiyun Lv,Ziqiang Cheng,Fang Liu,Ying Wang,Aiqin Zhou,Jianzhu Liu,Xiaona Zhao

Cellular & molecular biology letters 27:36 PubMed35562668

2022

RNF7 inhibits apoptosis and sunitinib sensitivity and promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Chengwu Xiao,Wei Zhang,Meimian Hua,Huan Chen,Bin Yang,Ye Wang,Qing Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SOCS1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (AB9870)

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (AB9870)

Immunocytochemistry - Anti-SOCS1 antibody (AB9870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (AB9870)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (44)

Product promise