Rabbit Recombinant Monoclonal SOCS1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications.
Images
![Western blot - Anti-SOCS1 antibody [EPR24290-356] (AB280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--western-blot-img4129.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOCS1 antibody [EPR24290-356] (AB280886)")
![Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (AB280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--immunoprecipitation-img4740.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (AB280886)")
![Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] (AB280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--flow-cytometry-intracellular-img16697.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] (AB280886)")
![Western blot - Anti-SOCS1 antibody [EPR24290-356] (AB280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--western-blot-img8076.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOCS1 antibody [EPR24290-356] (AB280886)")
Publications
Filter by
- Molecules (Basel, Switzerland) 28:2023Fruit Extract Alleviates DSS-Induced Ulcerative Colitis by Regulating Gut Microbiota, Inflammatory Mediators, and Cytokines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao-Yao Li,Yu Cui,Wan-Rong Dong,Tian-Tian Liu,Gao Zhou,Yu-Xin ChenPubMed 37570753
- Frontiers in pharmacology 13:10910902023The mechanism of oxymatrine on atopic dermatitis in mice based on SOCS1/JAK-STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianwei Han,Tianming Ma,Qiang Wang,Chunlin Jin,Yusheng Han,Guijun Liu,Hao LiPubMed 36703757
- The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 33:934-9442022MiR-222-3p Aggravates the Inflammatory Response by Targeting SOCS1 to Activate STAT3 Signaling in Ulcerative Colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Xia,Wenxia Bo,Jinli Ding,Yanqiu Yu,Jianning WangPubMed 35946880
- Bioengineered 13:2513-25242022Inhibition of in vivo and in vitro by antimicrobial peptides chromogranin A-N12 through microRNA-155/suppressor of cytokine signaling 1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohua Li,Qun Hu,Qiong Lin,Jianxiong Luo,Junping Xu,Lifang Chen,Liyu Xu,Xin LinPubMed 35034584
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Tested |
| Mouse | Not recommended | Expected | Tested | Not recommended | Expected |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Essential negative regulator of type I and type II interferon (IFN) signaling, as well as that of other cytokines, including IL2, IL4, IL6 and leukemia inhibitory factor (LIF) (PubMed:32499645, PubMed:33087723). Downregulates cytokine signaling by inhibiting the JAK/STAT signaling pathway. Acts by binding to JAK proteins and to IFNGR1 and inhibiting their kinase activity. In vitro, suppresses Tec protein-tyrosine activity (PubMed:9341160). Regulates IFN-gamma (IFNG)-mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:11278610, PubMed:11313480).
Alternative names
SSI1, TIP3, SOCS1, Suppressor of cytokine signaling 1, SOCS-1, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3, JAB, SSI-1, TIP-3
Recommended products
Rabbit Recombinant Monoclonal SOCS1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24290-356
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--western-blot-img4129.jpg "Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)")
Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:15580314, 26090662, 10490100).
Negative control: U266B1 (PMID: 15580314).
Exposure time: Lane 1: 48 seconds Lane 2-3: 3 minutes
All lanes: Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886) at 1/1000 dilution
Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate at 60 µg
Lane 2: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 60 µg
Lane 3: U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 60 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 22 kDa, 23 kDa
![Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (ab280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--immunoprecipitation-img4740.jpg "Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (ab280886)")
Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
SOCS1 was immunoprecipitated from 0.35 mg of KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab280886 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280886 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 ug
Lane 2: ab280886 IP in KARPAS-299 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab280886 in mouse eyeball tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 93 seconds
All lanes: Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
Predicted band size: 23 kDa
Observed band size: 23 kDa
![Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] (ab280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--flow-cytometry-intracellular-img16697.jpg "Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] (ab280886)")
Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U266B1 (human multiple myeloma B lymphocyte, left)/ KARPAS-299 (Human anaplastic large cell lymphoma, right) cells labelling SOCS1 with ab280886 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: U266B1 (PMID: 15580314).
![Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886), expandable thumbnail](https://content.abcam.com/products/images/socs1-antibody-epr24290-356-ab280886--western-blot-img8076.jpg "Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)")
Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 15580314, 26090662, 10490100).
Exposure time: 3 minutes
All lanes: Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886) at 1/1000 dilution
All lanes: Mouse thymus tissue lysate at 60 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
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