Anti-SOCS2 antibody [EPR2588(2)]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SOCS2 antibody [EPR2588(2)] (AB109245)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/100 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SOCS2 antibody [EPR2588(2)] (AB109245)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/150 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IP

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Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (AB109245)

Purified ab109245 at 1/70 dilution (2 μg) immunoprecipitating SOCS2 in K-562 whole cell lysate.
Lane 1 (input) : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg
Lane 2 (+) : ab109245 + K-562 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109245 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 22 kDa

All lanes:

Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

Predicted band size: 22 kDa

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• WB

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Western blot - Anti-SOCS2 antibody [EPR2588(2)] (AB109245)

All lanes:

Western blot - Anti-SOCS2 antibody [EPR2588(2)] (ab109245) at 1/2000 dilution

Lane 1:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

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